The core of this technology is reflected in the antibody incubation of this experimental process and the microfluidic technology of Chromium system. The core principles of these two processes are the basis for the simultaneous serialization of single-cell surface protein information and the barcoding of...
While single cell RNA sequencing (scRNA-seq) is invaluable for studying cell populations, cell-surface proteins are often integral markers of cellular function and serve as primary targets for therapeutic intervention. Here we propose a transfer learning
single cell n.单室电解槽 cell surface protein 细胞表面蛋白 single fluid cell 单液电池 single cell box girder 单元箱形梁 single cell isolation 单胞分裂 single cell animals 单细胞动物 相似单词 protein n. 蛋白质 a. 蛋白质的 single adj. 1. 单一的,单个的,仅一个的 2. 个别的,单独...
Surface protein imputation from single cell transcriptomes by deep neural networksdoi:10.1101/671180Zilu ZhouChengzhong YeJingshu WangNancy R. ZhangCold Spring Harbor Laboratory
(MSCs) are covered. Cells from young and aged BM occupied the same cell states in all individuals, whereas cell states in AML differed (Fig.1band see below). Importantly, the combined RNA and surface protein information provided higher resolution and revealed cell types that are not readily ...
1a). Each antibody is associated with a known oligo tag; thus, when cells are stained with a pool of tagged antibodies, each cell is bound with a combination of antibodies and their tags based on surface protein profile. Fig. 1: The DAb-seq workflow. a Bone marrow aspirates of patients...
Recent advances in single-cell technologies have enabled high-throughput molecular profiling of cells across modalities and locations. Single-cell transcriptomics data can now be complemented by chromatin accessibility, surface protein expression, adaptive immune receptor repertoire profiling and spatial informat...
High-throughput methods in single-cell genomics have made it possible to cluster thousands to millions of cells from a single experiment into distinct types based on whole transcriptome gene expression and cell surface protein data, sparking ambitious collaborations to profile every cell type in the ...
The purified oligos, now incorporating a 5′-NHS-ester, are added to a solution of antibody, where they react with amine residues on the antibody surface (Fig. 2a). Figure 2: DNA-antibody conjugation. (a) To enable single cell protein profiling, Abseq uses antibodies labeled with known ...
(Fig.3b, c, g). Both MIF and MK were also upregulated on protein level in NB tumors and cell lines compared to controls, and receptor expression was high on resting and activated monocytes (Fig.3h, i). To experimentally test the interactions of NB with myeloid cells, we measured ...