Single-cell multi-omics sequencing integrates omics information, whereas stereo-sequencing analyzes gene expression and spatiotemporal data simultaneously. This review discusses the protocols of single-cell sequencing for obtaining single cells from retina and accurate sequencing data. It highlights the ...
Although high-throughput single-cell sequencing has revealed the gene expression characteristics within the majority of physiological and diseased cells, the complexity of cells extends far beyond its scope. There is still a need to understand and decode the secrets of cells from multiple dimensions. ...
True single-cell resolution by Stereo-Seq and DNBSEQ-T7 A high-resolution spatiotemporal transcriptomic atlas of zebrafish embryogenesis can be constructed using the Stereo-seq kit S1 and the DNBSEQ-T7 sequencing platform. LEARN MORE Data Analysis Has Never Been Easier ...
Although high-throughput single-cell sequencing has revealed the gene expression characteristics within the majority of physiological and diseased cells, the complexity of cells extends far beyond its scope. There is still a need to understand and decode the secrets of cells from multiple dimensions. ...
enhancing our understanding of the pathogenesis and pathological changes in diabetic retinopathy. Additionally, single-cell sequencing is widely employed to analyze retinal organoids and single extracellular vesicles. Single-cell multi-omics sequencing integrates omics information, whereas stereo-sequencing analy...
i, Visium (Visium1: Mouse Brain Coronal Section 1; Visium2: Mouse Brain Coronal Section 2); ii, Stereo-seq; iii, STARmap (STARmap_mouse1) versus Slide-seq; iv, STARmap (STARmap_mouse1) versus MERFISH. CAST Projection preserves original single-cell resolution on mouse half brain and ...
Although single-cell gene expression profiling technologies have been developed, their application in characterizing cell subtypes has not been achieved yet. Here, we present scStereo-seq (single-cell spatial enhanced resolution omics sequencing) that enabled us to show the bona fide single-cell ...
sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal cells obtained from the fractionation of fresh hepatic tissue from five human livers. Using gene expression patterns, flow cytometry, and immunohistochemical examinations, we identify 20 discrete cell populations of ...
Single-cell RNA sequencing (scRNA-seq) affords unprecedented resolution in profiling cellular transcriptomics by simultaneously detecting the expression of thousands of genes on an individual cell basis. Tissue engineers can leverage scRNA-seq to comprehensively map healthy and perturbed (such as injured ...
single-cell sequencing; scRNA-seq: single-cell RNA sequencing; scDNA-seq: single-cell DNA sequencing; CNV: copy number variation; scATAC-seq: single-cell assay for transposase-accessible chromatin by sequencing; scChIP-seq: single-cell chromatin immunoprecipitation followed by sequencing; NGS: next...