What is single-cell RNA sequencing? Why use PacBio for single-cell RNA sequencing? WHAT IS THE MAS-SEQ TECHNOLOGY BEHIND KINNEX SINGLE-CELL RNA KIT? How is the Kinnex single-cell RNA kit different from MAS-Seq for 10x Single Cell 3’ kit? CAN KINNEX DATA DETECT CELL-TYPE SPECIFIC ISOFOR...
确定一下 cell 名的组成,构建好 metadata(行名) 和 count(列名) 矩阵能够对应 过滤掉外源 RNA (ERCC) 合并成为 seurat 对象 计算线粒体(MT),核糖体的 RNA(^RP[SL]) 比例并筛选数据 我们先处理第一个表达矩阵 # 这个是治疗前的第一个表达矩阵 # 导入数据 raw.data = read.csv('csv_files/S01_datafin...
ezSingleCell平台是一个集成的单细胞及空间组学数据分析工具,它通过直观的界面和强大的算法,如Seurat、Harmony、scVI和fastMNN,为实验科学家提供了从数据预处理、标准化、聚类、降维到高级分析的一站式服务,支持scRNA-seq、scATAC-seq、空间转录组学等多数据类型的分析,具备跨模块操作性,能够处理大规模数据集,并通过在...
Current best practices in single‐cell RNA‐seq analysis: a tutorialwww.embopress.org/doi/full/10.15252/msb.20188746 单细胞RNA测序(scRNA-seq )是一个飞速发展的领域,该技术帮助科研人员从一堆细胞(bulk)水平精细到单细胞(single-cell)水平研究基因的表达状态,从而可以更加精细的刻画科研对象的分子状态(例...
如果数据中存在批次效应，则应考虑这些效应，例如使用Seurat或Harmony，以避免因技术差异或批次效应而对细胞...
Since the first publication a decade ago describing the use of single-cell RNA sequencing (scRNA-...
of class Seurat # 15361 features across 4306 samples within 1 assay # Active assay: RNA (...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
Analysis of single cell RNA-seq datahemberg-lab.github.io/scRNA.seq.course/ 主讲人Vladimir Kiselev主编SC3和scmap两个single-cell RNA-seq数据分析包,其导师Martin Hemberg是剑桥教授。 有意思的是,现在R里处理single-cell RNA sequencing的packages主要分成两派:SingleCellExperiment派和Seurat派。 SingleCellEx...
The single-cell RNA data was processed using cellranger v.2.0.1 (https://www.10xgenomics.com/) and analyzed with the R package Seurat v.2.3.1 (https://satijalab.org/seurat/). Gene expression signatures were extracted using our SignatureAnalyzer algorithm available on github (https://github...