Mass spectrometry (MS), the most unbiased technique for single-cell proteomics, facilitates the identification and quantification of thousands of proteins, proteoforms, and post-translational modifications (PTMs) in individual cells. A successful single-cell proteomics workflow hinges on through...
preparation is not feasible for single-cell proteomics due to limited starting material and the shear number of cells to be analyzed. Automation addresses many of the short comings of single cell sample preparation. From cell sorting and isolation to preparation of proteins for mass spec...
(2016) Single-cell mass spectrometry for discovery proteomics: Quantifying translational cell heterogeneity in the 16-cell frog (Xenopus) embryo. Angew. Chem. 55, 2454 -2458Lombard-Banek, C.; Moody, S. A.; Nemes, P. Single-Cell Mass Spectrometry for Discovery Proteomics: Quantifying ...
Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS...
Fig. 1: Emerging applications of single-cell proteomics by MS. Single-cell proteomic measurements can define cell type and cell state clusters9, support pseudotime inference, link protein levels to functional phenotypes, such as phagocytic activity18, quantify protein covariation and apply it to study...
Single-cell assay is an ensemble of genomics, transcriptomics, proteomics and metabolomics to study a system at a single cell level.
Single-cell proteomics Proteins are essential macromolecules that are responsible for the main functional machinery within a cell, including regulations of gene expression, signaling pathway and catalytic reaction. Proteome measurements based on mass spectrometry (MS) have historically been limited to bulk ...
To fill this deficiency, we present a comprehensive database, namely Single-cell Proteomic DataBase (SPDB, https:// SPDB ), for general single-cell proteomic data, including antibody-based or mass spectrometry-based single-cell proteomics. Equipped with standardized data process and a user-...
Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS...
Single-cell proteomics: progress and prospects. Mol. Cell. Proteomics 19, 1739–1748 (2020). Article CAS Google Scholar Budnik, B., Levy, E., Harmange, G. & Slavov, N. SCoPE-MS: mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. ...