Conclusion: The creation of a LUAD single-cell atlas in our study offered new insights into its tumor microenvironment and immune cell interactions, highlighting the importance of key genes associated with exhausted CD8 T cells. These discoveries have enabled the development ...
In this study, we defined a set of genes correlated LUAD tumorigenesis and cell metabolism reprogramming named “MMR” according to LUAD scRNA-seq profiles. Machine leaning methods including Cox regression, random survival forest (RSF), CoxBoost, support vector machine (SVM), as well as Gradient...
三、LUAD multicellular spatial interactions We next assessed the relationship between immune populations and clinical or pathological variables by interrogating the frequency of individual cell types as a percentage of total cells within each image(Fig. 2a,b, Supplementary Tables 1 and 4 and Supplementa...
analyzed the potential cellular origins and driving factors of metastatic LUAD at the single-cell level, identifying relevant biomarkers and potential targeted drugs, which offer important insights into understanding the metastasis of LUAD (Wang et al., 2023). Thus, it can be observed that scRNA-...
Inducing angiogenesis involves the sprouting of established vessel beds and their maturation into an organized network2,3. Here we generate a comprehensive atlas of tumour vasculature at single-cell resolution, encompassing approximately 200,000 cells from 372 donors representing 31 cancer types. ...
This study aims to give a new perspective to the biomarkers in the lung adenocarcinoma (LUAD) brain metastasis, pathways involved and potential therapeutics. We performed a comprehensive single-cell level transcriptomic analysis on one LUAD patient with
Additionally, with some exceptions9,14, LUAD and LUSC were considered as a single entity thus hindering the investigation of specific hallmarks of the two cancer types which are radically distinct both at the molecular and pathological level. While single-cell RNA-seq (scRNA-seq) can identify ...
A single cell map of the immune microenvironment in human PDAC 人类PDAC免疫微环境的单细胞图谱 我觉得都这儿就是描绘了一下肿瘤内细胞的一个分布情况。 单细胞测序 细胞间存在明确界限: - immune (CD45+,PTPRC)epithelial/cancer (EpCAM+,EPCAM) - stromal (CD10+,MME,fibo or CD31+,PECAM1,endo) ...
[5,6]. Single-cell RNA sequencing (scRNA-seq) technologies enable the evaluation of thousands of cells within a single sample, thereby revealing the heterogeneity of tumor cells and the subtle nuances of the complex TME [7]. The development of LUAD is often considered to progress from ...
Exploring the biology of LUAD and identifying new biomarker and therapeutic targets options are essential. Method: We performed single-cell RNA sequencing (scRNA-seq) of 6 paired primary and adjacent LUAD tissues, and integrative omics analysis of the scRNA-seq, bulk RNA-seq and whole-exome ...