While gene expression is more accurately measured by RNA sequencing (RNA-seq), ATAC-seq can explain the mechanism behind how gene expression is regulated or why it might be different between two cell types or conditions. 看完了原理,再来看处理细节: 10x - Cell Ranger ATAC Algorithms Overview pea...
Single Cell Multiome ATAC + Gene Expression assay generates two single cell library types, ATAC (assay for transposase chromatin accessibility) library and 3' Gene Expression library, from the same GEM well.Sample: A nuclei suspension extracted from a single biological source (blood, tissue, etc)...
For the sample labeled as ‘10x_complex’ we used a modified protocol from the Nuclei Isolation from Complex Tissues for Single-Cell Multiome ATAC + Gene Expression Sequencing Protocol (CG000375) from 10x Genomics. Briefly, a ~1-cm3 frozen piece of mouse cortex tissue was transferred to 0....
Chromium Next GEM Single Cell Multiome Reagent Kit A, 16 rxns PN-1000282 Library Construction Kit, 16 rxns PN-1000190 Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 4 rxns PN-1000285, includes: Chromium Next ...
Prior to single cell multiome ATAC and gene expression sequencing, nuclei isolation was performed by resuspending PBMCs in 100 µL of cold lysis buffer containing 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% Nonidet P40, 0.01% digitonin, 1% BSA, ...
measurement of multiple molecular modalities at single-cell resolution, including single-cell gene expression (GEX) profiling (single-cell RNA-sequencing (scRNA-seq)) [1, 2] and single-cell chromatin accessibility (Assay for Transposase-Accessible Chromatin; ATAC) profiling (scATAC-seq) [3, 4]. ...
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The emergence of high-throughput approaches, combining ATAC-seq and RNA-seq from the same cell, with the potential to integrate protein expression and cell-lineage tracing (e.g., DOGMA-seq), has enormous potential in unravelling the dynamic interactions underpinning molecular cell identity during ea...
Cell Ranger ARC'spipelines analyze sequencing data produced from Chromium Single Cell Multiome ATAC + Gene Expression. The Cell Ranger ARC pipeline can only analyze Gene Expression and ATAC data together. It must not be used to analyze Gene Expression or ATAC alone. ...
Chromium Epi Multiome ATAC performs direct measurements of 3' gene expression and chromatin accessibility from the same cell.