Type 1 diabetes (T1D) is an autoimmune disease in which immune cells destroy insulin-producing beta cells. The aetiology of this complex disease is dependent on the interplay of multiple heterogeneous cell types in the pancreatic environment. Here, we pr
cell metabolism as much as possible.Mass spectrometry is a promising tool for single-cell metabolomics and its innovation provides rich information of metabolites for single-cell analysis.In this mini-review,the recent new advances of single-cell metabolomics based on microfluidics and mass spectrometry...
Peer review informationPrimary Handling Editor: Pooja Jha. Publisher’s noteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. About this article Cite this article He, X., Memczak, S., Qu, J.et al.Single-cell omics in ageing: ...
Single-cell assay is an ensemble of genomics, transcriptomics, proteomics and metabolomics to study a system at a single cell level.
5b), indicating that its absorption and metabolism of water had reached equilibrium. At the highest UV dose (200 mJ/cm2), the C-D ratios of the four strains were at a comparably low level after they reached saturation within 1 h. Single cell activities were also measured using D2O-...
Papers of particular interest, published within the period of review, have been highlighted as: 1 A. Ali, S. Davidson, E. Fraenkel, I. Gilmore, T. Hankemeier, J.A. Kirwan, A.N. Lane, I. Lanekoff, M. Larion, L.I. McCall, et al. Single cell metabolism: current and future trend...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
and mechanisms of therapeutic resistance in MM. In this review, we summarize the recently developed SCS methodologies and latest MM research progress achieved by single-cell profiling, including information regarding the cancer and immune cell landscapes, tumor heterogeneities, underlying mechanisms and bio...
The tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) is a complex ecosystem that drives tumor progression; however, in-depth single cell characterization of the PDAC TME and its role in response to therapy is lacking. Here, we perf
. The goal is to slow or stop cell metabolism and maintain the tissue as it was isolated. The sample cryopreservation of PCa can be divided into two methods: (1) the fresh tissue can be dissociated into single-cell suspensions, and the cell suspensions were frozen; (2) Fresh tissues can...