今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-...
Bulk RNA数据基于细胞群体水平获得组织的平均特性,反映细胞群体的平均差异,而Single-cell RNA通过单个细胞的mRNA进行转录组分析,识别不同细胞亚群的细胞类型、状态和谱系,绘制细胞图谱、鉴定细胞异质性,可以精准解析细胞亚类功能及对疾病的影响[...
早!今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-cell and bulk RNA-sequencing》。作者通过单细胞和bulk RNA测序的联合分析...
今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-cell and bulk RNA-sequencing》。作者通过单细胞和bulk RNA测序的联合分析,鉴...
We obtained single-cell transcriptomes for 144,878 cells after quality control. Thirteen main cell clusters with the expression of known marker genes were identified including epithelial cells, monocytes, macrophages, dendritic cells (DC), natural killer (NK) cells, CD4+ T cells, regulatory T ...
We identify and classify neurons based on novel subtype-specific marker genes using single-cell qRT-PCR and IHC to validate subtypes derived from RNA-sequencing. Lastly, functional Ca2+-imaging was conducted on colonic sensory neurons to demonstrate subtype-selective differential agonist activation....
Inference of cell–cell communication from single-cell RNA sequencing data is a powerful technique to uncover intercellular communication pathways, yet existing methods perform this analysis at the level of the cell type or cluster, discarding single-cell-level information. Here we present Scriabin, a...
已经开发了几种资源来简化此方法，例如CellMarker（28），PangaloDB（29）和ImmuCellDB（30），它们利用...
However, computational methods for analyzing single-cell transcriptomics still have room for improvement especially in dimension reduction, cell clustering, and cell–cell communication inference. Herein, we propose a versatile method, named DcjComm, for comprehensive analysis of single-cell transcriptomics...
To further analyze the impact of the reference batch, we performed cell type marker gene detection and subsequent enrichment using cell type-specific gene sets (PanglaoDB [34]). We could see an increase in the expected cell type enrichment scores for nearly all reconstructions and cell types comp...