Then we performed RNA Library construction with Chromium Single Cell 3’ Reagent Kits v2 following the default process(www.10xgenomics.com). The library was processed on the Illumina HiSeq4000 platform for sequencing with 150 bp paired-end reads. Data processing of single-cell RNA-seq from ...
cycles and cleaned up using SPRIselect beads (Beckman). Samples were quantified on an Invitrogen Qubit 4 Fluorometer. cDNA libraries were prepared according to the Single Cell 3 Reagent Kits v2 guide with the appropriate choice of PCR cycle number based on the calculated cDNA concentration. Final ...
Single cells were purified by FACS sorting before library preparation, and single-cell libraries were prepared with the Chromium Single Cell 3’ Reagent Kits v2 (10x Genomics) as per manufacturer’s instructions. Briefly, sorted cells in suspension were first prepared as gel beads in emulsion (GEM...
Technical Note - Biological & Technical Variation in Single Cell Gene Expression Experiments The Chromium Single Cell 3′ v2 Reagent Kits protocol (Document CG00052) produces Single Cell 3′ short-read sequencer compatible libraries. Technical and biologica...
Chromium Next GEM Single Cell 3ʹ Kit v3.1, 16 rxns PN-1000268 Chromium Next GEM Single Cell 3ʹ Kit v3.1, 4 rxns PN-1000269 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96...
Chromium Single Cell 3ʹ Library & Gel Bead Kit v2 10x Genomics Cat #: PN-120237 Chromium Single Cell A Chip Kits 10x Genomics Cat #: PN-120236 Chromium i7 Multiplex Kit 10x Genomics Cat #: PN-120262 SPRIselect Reagent Kit Beckman Coulter Cat #: B23318 Multiplex Fluorescent v2 system AC...
Single nucleus RNA-seq libraries were generated using Single Cell 3′ Reagent Kits v2 (10xGenomics) according to the manufacturer’s protocol. Briefly, cells, reagents (10xGenomics, PN-120237), gel beads (10xGenomics, 220104), and partitioning oil (10xGenomics, 220088) were loaded into the Chr...
enabling large-scale parallel single-cell gene expression studies. The gene expression (GEX) libraries were prepared using the Chromium 3’V2/3 reagent kits (10x Genomics, 120237 and 1000075). Brieflfly, gel bead-in-emulsions (GEMs) were generated and barcoded by loading single-...
Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide Universal 3' Gene Expression User Guide,CG000399 CG000399_ChromiumNextGEMSingleCell3'_LT_v3.1_RevB.pdf View and download FOR USE WITH: Chromium Next GEM ...
Following the Single Cell 3’ Reagent Kits v2 User Guide (CG0052 10x Genomics)34, a single-cell RNA library was generated using the GemCode Single-Cell Instrument (10x Genomics, Pleasanton, CA, USA) and Single Cell 3’ Library & Gel Bead Kit v2 and Chip Kit (P/N 120236 P/N 120237...