Chromium Next GEM Single Cell 3ʹ Kit v3.1, 16 rxns PN-1000268 Chromium Next GEM Single Cell 3ʹ Kit v3.1, 4 rxns PN-1000269 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96...
cycles and cleaned up using SPRIselect beads (Beckman). Samples were quantified on an Invitrogen Qubit 4 Fluorometer. cDNA libraries were prepared according to the Single Cell 3 Reagent Kits v2 guide with the appropriate choice of PCR cycle number based on the calculated cDNA concentration. Final ...
The sequencing libraries were constructed with the Chromium Single Cell 3′ Reagent Kits v.2 (10 × Genomics) and sequenced with the NextSeq 500/550 High Output v2 kits (150 cycles). RNA abundance and cell type identification Intronic and exonic counts were obtained using kb-python (v...
Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for Cell Surface Protein User Guide, v3 Chemistry with Feature Barcoding technology, Last Modified on February 18, 2020, Permalink CG000185_ChromiumSingleCell3'v3_FeatureBarcoding_CellSurfaceProtein_UG_...
Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide Universal 3' Gene Expression User Guide,CG000399 CG000399_ChromiumNextGEMSingleCell3'_LT_v3.1_RevB.pdf View and download FOR USE WITH: Chromium Next GEM ...
Can I resuspend the cells in buffers medium other than PBS before cell collection Do the cells have to be sorted in to a specific reagent Can frozen cells be used? Does the NEBNext Single Cell/Low Input RNA Library Prep Kit include adaptors and primers?
Table S2. Detailed Clinical Information of Patients from Whom Samples Were Studied by High-Throughput Single-Cell RNA Sequencing (10x Chromium), Related to Figures 2, 3, 4, 5, and 6A Table S3. Quality Control and Cells Filtered Out during Quality-Control Steps on Single-Cell RNA Sequencing ...
highest contribution in the reticulum (Fig.6E). Furthermore, we investigated the expression levels of these ligands and receptors in each cell subtype of the forestomach mucosa. Except for granular cell 3 in the rumen and undefined epithelial cells in the omasum, all other cell subtypes ...
Depending on your experimental goals, additional Library Construction Kits (PN-1000190) may be required. Please clickherefor further guidance. FOR USE WITH: Chromium Next GEM Single Cell 5' Kit v2, 16 rxns PN-1000263 Chromium Next GEM Single Cell 5' Kit v2, 4 rxns PN-1000265 ...
CG000209_Chromium_NextGEM_SingleCell_ATAC_ReagentKits v1.1_UserGuide_RevG.pdfView and download FOR USE WITH: CG000169: Nuclei Isolation for Single Cell ATAC Sequencing CG000212: Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing Chromium Next GEM Single Cell ATAC Library &...