cycles and cleaned up using SPRIselect beads (Beckman). Samples were quantified on an Invitrogen Qubit 4 Fluorometer. cDNA libraries were prepared according to the Single Cell 3 Reagent Kits v2 guide with the appropriate choice of PCR cycle number based on the calculated cDNA concentration. Final ...
The sequencing libraries were constructed with the Chromium Single Cell 3′ Reagent Kits v.2 (10 × Genomics) and sequenced with the NextSeq 500/550 High Output v2 kits (150 cycles). RNA abundance and cell type identification Intronic and exonic counts were obtained using kb-python (v...
The single-cell suspensions were adjusted to a concentration of 1500 cells/µl, and a total of 22,000 cells/sample were loaded onto a chromium controller utilizing Chromium Single-Cell 3′ Reagent Kits (v3 chemistry). Samples were sequenced using Illumina Nova S6000 instruments. Single-cell RN...
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) Single Cell Gene Expression User Guide, CG000315 CG000315_ChromiumNextGEMSingleCell3-_GeneExpression_v3.1(DualIndex)_RevF.pdf View and download FOR USE WITH:
CG000185_ChromiumSingleCell3'v3_FeatureBarcoding_CellSurfaceProtein_UG_Rev D.pdf FOR USE WITH:Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075 Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 4 rxns PN-1000092 Chromium Single Cell 3ʹ Feature Ba...
Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide Single Cell Gene Expression User Guide,CG000399 CG000399_ChromiumNextGEMSingleCell3'_LT_v3.1_RevB.pdf View and download FOR USE WITH: Chromium Next GEM S...
Can I resuspend the cells in buffers medium other than PBS before cell collection Do the cells have to be sorted in to a specific reagent Can frozen cells be used? Does the NEBNext Single Cell/Low Input RNA Library Prep Kit include adaptors and primers?
Table S2. Detailed Clinical Information of Patients from Whom Samples Were Studied by High-Throughput Single-Cell RNA Sequencing (10x Chromium), Related to Figures 2, 3, 4, 5, and 6A Table S3. Quality Control and Cells Filtered Out during Quality-Control Steps on Single-Cell RNA Sequencing ...
highest contribution in the reticulum (Fig.6E). Furthermore, we investigated the expression levels of these ligands and receptors in each cell subtype of the forestomach mucosa. Except for granular cell 3 in the rumen and undefined epithelial cells in the omasum, all other cell subtypes ...
CG000207_ChromiumNextGEMSingleCellV(D)J_ReagentKits_v1.1_UG_Rev G.pdf View and download Depending on your experimental goals, additional Single Cell 5' Library Construction Kits (PN-1000020) may be required. Please clickherefor further guidance. ...