For this analysis, the adult epicardial subclusters of Adult_Epicardium_Proliferating and Adult_Epicardium_FB-like were omitted as there were fewer than three cells in each (n = 0 and 2, respectively). The numbers of significant interactions for the remaining epicardial clusters were then ...
The dissociated single cells from primary fibroadenoma tissues or normal tissues were resuspended in 100 μl of cell staining buffer (Biolegend, Cat.# 420201) and stained with Zombie Red™ (Biolegend, Cat.# 423109) to distinguish live cells for 30 min at room temperature according to th...
cross-validation where the non-proliferating epicardial clusters were predicted with high accuracy (Extended Data Fig.5). These results reflect the separation of Epicardium_Mesothelial and Epicardium_FB-like populations found in vivo. Both populations appeared to stem from a population predicted as fetal...
Single-use biobags with sendors DO, pH, CO2 and t 优质文献 相似文献 参考文献 引证文献Microfabrication of single-use plastic microfluidic devices for high-throughput screening and DNA analysis Modern drug discovery and genomic analysis depend on rapid analysis of large numbers of samples in parallel...
Water soluble organometallic carbonyl clusters are bio-compatible, stable and reliable high-contrast photoacoustic contrast agent (PACA). But they have limited application and efficacy due to their absorption in the visible region which ... A Bag - 《Rsc Advances》 被引量: 3发表: 2015年 Abstract...
DAB visualization (ZSGB-BIO, ZLI-9018) was used to detect the biotinylated antibodies. For immunofluorescence staining, the slides were incubated with primary antibodies overnight at 4 °C. This was followed by incubation with secondary antibodies, and color development was carried out using a ...
Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat...
Cell Biosci. 2019;9(1):53. Article PubMed PubMed Central Google Scholar Pilarczyk M, Fazel-Najafabadi M, Kouril M, Shamsaei B, Vasiliauskas J, Niu W, et al. Connecting omics signatures and revealing biological mechanisms with iLINCS. Nat Commun. 2022;13(1):4678. Article CAS Pub...
The primary antibodies used were rabbit anti-KI67 (1:100, ab15580, Abcam), mouse anti-AMH (1:30, MCA2246T, BioRad), rabbit anti-Troponin I (H-170) (1:100, sc15368, Santa Cruz), goat anti-VASA/DDX4 (1:200, AF2030, R&D), mouse anti-Cytokeratin (1:100, M351501, DAKO), ...
Cancer-associated fibroblasts (CAFs) are a diverse cell population within the tumour microenvironment, where they have critical effects on tumour evolution and patient prognosis. To define CAF phenotypes, we analyse a single-cell RNA sequencing (scRNA-se