We addressed our aims using single-cell RNA sequencing (scRNA-seq) to isolate epicardial cells in silico and mitigate biases from sorting and selection. Although scRNA-seq data of both adult and fetal hearts have been generated and analyzed independently, no attempt has been made to combine them...
A benchmark of batch-effect correction methods for single-cell RNA sequencing data. Genome Biol. 21, 12 (2020). Article CAS Google Scholar Welch, J. D. et al. Single-cell multi-omic integration compares and contrasts features of brain cell identity. Cell 177, 1873–1887.e17 (2019). ...
vascularized composite and reconstructive transplantationThis editorial comments on a landmark case of single‐stage long segment tracheal transplantation (Genden et al., page 3421 ).doi:10.1111/ajt.16776Simran K. RandhawaDivision of Cardiothoracic Surgery Washington University School of Medicine St. Louis...
The last portion of this division generates two DP algorithms, one of which is employed on finding the solution for the Pm|p(xj)|∑wjUj+∑G(xj) problem with O(n2tmaxmax{bj−aj}) complexity. A second DP algorithm is applied to the single machine problem generated via decomposition to ...
The ability to measure simultaneously multiple genes in the same cell is necessary to resolve the great diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can......
Benchmarking high fidelity single-shot readout of semiconductor qubits Determination of qubit initialisation and measurement fidelity is important for the overall performance of a quantum computer. However, the method by which it is calculated in semiconductor qubits varies between experiments. In this pa...
Here, we document the development and benchmarking of an Illumina compatible dual-indexed library structure for the inDrop scRNA-seq platform that builds upon the widely-used, commercially available V2 gel beads in a manner independent of the cell barcodes incorporated into the library. We demonstr...
The metabolism and reproduction of plants depend on the division of labors among cells. However, cells with various functions are often studied as a bulk where their specificities were diluted. Here, we apply single-cell RNA sequencing to the aerial part of rice seedlings growing in various envir...
Single-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) has emerged as a powerful tool for dissecting regulatory landscapes and cellular heterogeneity. However, an exploration of systemic biases among scATAC-seq technologies has remained absent. In this study, we benchmark the...
Last, we include two methods as negative (Neg) controls in our benchmarks, for which we expect poor performance: the raw untransformed counts (y) and the raw counts scaled by the size factor (y / s). Conceptual differences A known problem for variance-stabilizing transformations based ...