Cryo-EM sample preparation and data collection Aliquots (3.0 μL) of purified SGIV were applied to freshly glow-discharged holey carbon-coated copper Quantifoil grids (R2/1 in 300 mesh). The grids were blotted for 3 s and then flash-plunged in liquid ethane using a Vitrobot Mark IV ...
The dramatic frequency difference of VKORC1, which has become an important consideration in medical practice, is likely due to a hitchhiking effect of selection within the locus, such as the reported selection on PRSS53 (Szpak et al., 2018). For many loci, the critical region of selection ...