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AS interferes with most of the biochemical assays like total reducing sugar assays, colorimetric protein estimation assays and SDS-PAGE. Dialysis step is thus an unavoidable hindrance after AS precipitation16. The AS precipitation profile of a new/novel protein is usually unknown. Hence, optimization...
Apoptosis Caspase-3 Flow cytometry Förster resonance energy transfer Green fluorescent protein Paracrine signaling 1. Introduction High-throughput cell analysis by using flow cytometry is gaining in importance as a technique for quantification of cellular events and for drug screening. The technique has...
The blue curve is the real-time projection of K with τ fixed to be March 1, 2016 (vertical blue dashed line). The green curve is the real-time projection of K with τ fixed to be April 1, 2016 (vertical green dashed line). The shading area represents the 95% CI Full size image...
// // Some useful references: // http://www.skyfree.org/linux/references/ELF_Format.pdf // https://eli.thegreenplace.net/2011/08/25/load-time-relocation-of-shared-libraries/ static int cr_dl_header_handler(struct dl_phdr_info *info, size_t, void *data) { CR_ASSERT(info && data...
However, performing the same PCR protocol using freshly grown cells preboiled in 0.1% SDS or Triton X-100 yielded better, but inconsistent results (not shown). To obtain reliable results, we designed a PCR protocol with a linearly decreasing annealing temperature going from 1~ below the ...
Distribution of intrinsic solubility values, log10 S0, in the training database set (green upper trace, scaled to the left vertical axis) and the test set (red lower trace, scaled to the right vertical axis) of newly approved drugs. The bell-shaped curves illustrate that the newly-approved...
We chose two DNA fragments derived from the coding region of the green fluorescent protein (GFP) gene that had been interrupted by Cas9 and repaired by non-homologous end joining (one with a 1-bp deletion; the other with a 29-bp deletion). These two fragments were mixed together in equal...
As well, because of tissue autofluorescence in the blue-green spectrum (Figure S1), imaging of fluorescence from the most common reporters like GFP (that emit in that spectrum) is de facto limited to transgenic lines with high expression—the only examples reported so far in the literature. ...
The two structural units are colored green (unit 1) and red (unit 2) in each protein and are also shown superimposed onto the three-dimensional structure of protein L (left) and ubiquitin (right). We thank David Baker for providing the plasmid encoding monomeric Y47W protein L. We also ...