The bacterial staining with acridine orange/ethidium bromide dyes as examined through fluorescence microscopy also substantiated with the results of membrane permeability of bacteria. Thus, the investigated microemulsion system will act as the potential oral drug delivery system with beneficial efficacy in ...
Here we mainly use the Alzheimers related -amyloid peptide as a model system, but the technique can be easily implemented for screening inhibitors relevant for other conformational diseases simply by changing the recombinant amyloid protein target. Indeed, we show that this methodology can be also ...
Rieske non-heme iron dioxygenases (ROs) catalyze stereo- and regiospecific mono- or dihydroxylation reactions and naturally play a vital role in pathways for bacterial degradation of aromatics and recycling of organic matter in the environment [5], [6], [7]. As the first step of such ...
In other words, this technique monitors bacterial activity and simultaneously confers spectroscopic specificity. In this work, we report the discovery of simple SERS spectroscopic biomarkers for bacterial AST and deter- mination of minimum inhibitory concentrations (MICs). The schematic comparison between ...
This technique can be easily adapted to other neuronal subtypes owing to its short duration in design, nine days from start to finish, and limited manipulation during screening. Future efforts will focus on improving scalability of iNs for more complex assays that measure aspects of neuronal ...
Bacterial colonies containing the desired recombinants can be readily identified using the familiar blue/ white screening technique for the presence or absence of β-galactosidase [12, 13]. Results Rationale of the cloning system I sought to develop a procedure that would facilitate the cloning of ...
All Plates and broth are inspected at least once daily for bacterial growth. Species identification relied on MALDI-TOF technique combined with classical bio- chemical identification methods. NAAT analysis Nucleic acid extraction (RNA and DNA) was performed on a QIAsymphony SP/AS instrument with the...
According to the recommendations of the WHO, to replace the “gold standard”, bacterial culture, a serological test must possess a sensitivity of over 80% and specificity of over 95% [5]. In attempts to develop a serological test, mycobacterium antigens, such as 38 kDa antigen [6], 30 ...
We checked amplification on 1% agarose gels using ethidium bromide staining. Mite-associated bacteria: 16S sequencing and analysis Second, to assess infection with other potential reproductive parasites, we performed a 16S rDNA metagenomic survey of the bacterial community in and on the parental ...
This will rule out allergic conjunctivitis, allergy or another type of conjunctivitis such as bacterial or viral, Dr. Nichols says. Of course, a patient can have allergy and dry eye simultaneously. “If this is the case, you need to sort through what is the major element leading the patient...