qiime rescript dereplicate \--i-sequences silva-138-ssu-nr99-seqs-filt.qza \--i-taxa silva-138-ssu-nr99-tax.qza \--p-rank-handles 'silva' \--p-mode 'uniq' \--o-dereplicated-sequences silva-138-ssu-nr99-seqs-derep-uniq.qza \--o-dereplicated-taxa silva-138-ssu-nr99-tax-...
ln -s silva-138-99-tax.qza silva-138-ssu-nr99-tax.qza ln -s silva-138-99-seqs.qza silva-138-ssu-nr99-seqs.qza 之后就可以参考这里建立自己的分类器:https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494 ...
qiime rescript dereplicate\--i-sequencessilva-138-ssu-nr99-seqs-filt.qza\--i-taxasilva-138-ssu-nr99-tax.qza\--p-rank-handles'silva'\--p-mode'uniq'\--o-dereplicated-sequencessilva-138-ssu-nr99-seqs-derep-uniq.qza\--o-dereplicated-taxasilva-138-ssu-nr99-tax-derep-uniq.qza 4...
1. 导入序列和分类文件 qiime tools import \ > --type 'FeatureData[Sequence]' \ > --input-path SILVA_132_QIIME_release/rep_set/rep_set_16S_only/99/silva_132_99_16S.fna \ > --output-path 99_otus.qza qiime tools import \ > --type 'FeatureData[Taxonomy]' \ > --input-format Head...
From there you can proceed with your own curation either within QIIME 2 or externally if you wish to export. As of now, get-silva-data supports the following versions of SSU and LSU: 128, 132, 138, 138.1. We also leave the door open to parse all the other versions, but these may ...