Yet, amidst the chaos, there’s beauty in the messiness of it all—the sticky ice cream fingers, the grass-stained knees, and the heartfelt laughter that echoes through our days. So, here’s to embracing the summer spirit with open arms and finding solace in the warm embrace of our ...
F-actin and nuclei were then stained by incubating cells with Alexa Fluor 568-conjugated Phalloidin (A12380, Invitrogen, Thermo Fisher) at a 1:100 dilution and with 4′,6‐diamidino‐2‐phenylindole (DAPI) (AnaSpec Inc., Fremont, CA) at a 1:10,000 dilution for 30 min and 5 min ...
cagnagella has a rather grid-like character, occasionally connected by a tuft of adhesive (Fig. 2a–d). The relatively low adhesive content is also evident in cross sections stained with toluidine blue and examined by light microscopy (Fig. 2e, f). Based on the different staining intensity,...
Filature or Reeling.—When the cocoons have been gathered the chrysalides they contain are killed either by dry heat or by exposure to steam. All cocoons stained by the premature death of the chrysalides (ehiques), pierced cocoons, double cocoons, and any from other causes rendered unreelabl...
5 µm sections were stained with haematoxylin and eosin (H&E), and alcian blue. The histological damage was evaluated by a blinded pathologist observer, according to a previously reported histological score (Camuesco et al., 2012), taking into account presence of ulceration, infiltration, ...
7B). By week 6, the red fluorescence was broader and more intense (Fig. 7D). The number of nucleus was clearly increased compared to at week 3 (Fig. 7E). Considering both the labeled cells and the images of DAPI-stained nucleus, it could be proved that NP cells could grow and ...
After 21 days of culture, cells were washed two times with D-PBS, fixed for 30 minutes at room temperature with 4% PFA and stained with Oil Red O (Sigma-Aldrich) [26]. Osteogenic differentiation was obtained by incubating cells in osteogenesis differentiation medium (ODM) for 21 days. ...
Samples were incubated for 3.5 h, then the cells on the underside of the filter were fixed, stained with 0.5% crystal violet (Sigma Aldrich, Scotland, UK) and counted under a microscope. The in vitro scratch wound healing assays were performed by seeding cells into 12-well plates at ...
a Growth of human umbilical vein endothelial cells (HUVECs) on the self-assembled (at 3000 rpm for 5 min) and natural SF fibers, respectively (cell nuclei and fibers were stained in blue where the nuclei with bright-blue dots are indicated by the white dotted ovals. F-actin was stai...
. To estimate the cell viability before and after adding microspheres, 0.5 ml of above cell suspension (2×105cells/ml) was mixed with 0.1 ml of 0.4% Trypan Blue Stain (Sigma-Aldrich), and the mixture was added to fill a hemocytometer for cell counting. Non-viable cells were stained ...