The invention relates to a new signal peptide from Bordetella pertussis with the amino acid sequence M K K W F V A A G I G A G L L M L S S A A and to particularly suitable expression vectors with whose aid such signal sequences can be found and/or evaluated.STEFAN KNAPP...
1991 . The extensin signal peptide allows secretion of a heterologous protein from protoplasts . Gene 99 : 95 – 100 .De Loose M, Gheysen G, Tire´ C, Gielen J, Villaroel R, Genetello C, van Montagu M, Depicker A, Inze´ D. 1991. The extensin signal peptide allows secretion ...
Signal peptides (SP) are short peptides located in the N-terminal of proteins, carrying information for protein secretion. They are ubiquitous to all prokaryotes and eukaryotes. SPs have been of special interest in several scientific and industrial fields, including recombinant protein production, dis...
Secretion of heterologous proteins intoEscherichia colicell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an important role in targeting proteins for translocation acr...
This signal peptide (secrecon) directs strong protein secretion and expression. We further ranked the signal strengths of the signal peptides of the known human secretory proteins by SP-HMM bit scores. The applications of high-bit scoring HMM signals in recombinant protein production and protein ...
Addition of the first six amino acid residues of mature SceA to the signal peptide did also not lead to secretion of hGH. It was concluded that the properties of the mature protein domains determine whether a propeptide is required for secretion or not. The Lip propeptide could be efficiently...
Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae . Microb Cell Fact (2012) 11 :97.10.1186/1475-2859-11-97 [ Cross Ref ]Klatt S, Konthur Z (2012) Secretory signal peptide modification for optimized antibody-fragment expression-...
The two known isoforms of IL-15 contain either a long signal peptide (LSP) or a short signal peptide (SSP), and are produced by alternatively spliced transcripts. It has been proposed that SSP IL-15 remains exclusively intracellular, and its function is unclear. In this study, we show that...
Expression and secretion of a single-chain sweet protein monellin in promoter and signal peptide 来自 Semantic Scholar 喜欢 0 阅读量: 36 作者:Z Chen,H Cai,Z Li,X Liang,X Shangguan 摘要: The sweet protein monellin gene was expressed in promoter and signal peptide sequence. A 294-bp DNA ...
Signal peptides (SPs) are short amino acid sequences that control protein secretion and translocation in all living organisms. As experimental characterization of SPs is costly, prediction algorithms are applied to predict them from sequence data. However, existing methods are unable to detect all kn...