Synchronized worms were collected from plates, washed at least three times with M9 buffer, and then resuspended with SDS loading buffer (100 mM Tris-HCl pH 6.8, 4% SDS, 20% glycerol, 10% β-mercaptoethanol, 0.004% bromophenol blue) and boiled at 100 °C for 15 min. Lysates contai...
(A–B) Representative trajectories of four diffusion states (Immobile: blue, Slow: yellow, Medium: green, Fast: red) of MOR, Gβ and GRK3 molecules in the Gβ1- (A) or Gβ5-expressing (B) cells. scale bar: 500 nm. (C–D) MSD–Δt plots of MOR, Gβ and GRK3 molecules in...
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Representative images of protein microarray showed positive control (blue arrow), negative control (yellow arrow) and CAMKK2 spot (red arrow) on the enlarged images. B Molecular docking predicted the potential binding sites of bufalin and CAMKK2. C Western blot analysis of CAMKK2 expression in...
Confocal images of mutant or wild-type XX embryos at approximately the 100-cell stage, costained with antibodies against the dosage compensation proteins DPY-27 (green) and SDC-3 (red) and with DAPI (blue), a DNA-intercalating dye. (A) In wild-type XX embryos, DPY-27 and SDC-3 locali...
b, Superimposition of Sec61–KZR-8445 complex with yeast α-factor signal peptide (red) engaged with Sec61 (PDB: 7AFT, blue). c, Sec61/KZR-8445 superimposed with a yeast α-factor signal peptide showing the solvent-excluded surface of the translocon. Indicated in gray is a putative route...
Cell lysates were immunoprecipitated with anti-Flag M2 agarose beads, and immunoprecipitates of anti-Flag-CSN6 were separated using SDS–PAGE and stained with Coomassie Brilliant Blue (left). The protein bands were retrieved and analyzed by mass spectrometry (right). The position of FASN and Flag...
Dye used at 1:1500 dilution. Marker shown is PPIB in red. Nuclei are counterstained with DAPI (blue, Cat. 5748, Tocris). Plan Your Experiments Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our ...
KdpD is a four-spanning membrane protein that has two large cytoplasmic domains at the amino- and at the carboxyterminus, respectively. During its biogenesis KdpD binds to the signal recognition particle (SRP) of Escherichia coli that consists of a 48-kD
aSchematic of 6-week-old mouse coronal brain section indicating the injection site (red arrow) and analysed area (blue rectangle).bAfter 7 weeks post transplantation polarized rosette structures (arrowheads) of transplanted cNESCs (H1JA, CA1J) contain proliferating KI67 positive human cells. The ...