(2007): F: 5′ATGCCAGCTTATCTGTTT-3′; R: 5′AAAGCAATTTTCACAGTCC-3′. A total of 5 μl (10 ng/μl) genomic DNA was amplified in a reaction mixture containing 12.5μl iQ SyBr Green Supermix (BioRad), 1 μl (7 μM/μl) of each of forward and reverse primers, and made ...
To further investigate the impact of the gp120 V2 PNGS in aa160 of HIV-1 R5 viruses for efficient DC-SIGN use we set up competition assays. On the basis of our previous findings showing that R5 isolates obtained before and after AIDS onset display varying ability to infect target cells in...