For neonatal rat cardiomyocytes (NRCMs), cells were fixed for 1 h at 4 °C in 4% glutaraldehyde with 0.1 mM cacodylate buffer and for 1 additional hour at room temperature in 1% osmium tetroxide in the same buffer. The fixed samples were dehydrated using a graded ethanol series and embedde...
for 1 hour. Lane U contains an amplified fragment which was not cut with exonuclease III. As shown, all digested amplicons produced ligation products demonstrating the premise of self-ligation. Self ligation would be helpful if one were to attempt to use this methodology in a gene shuffling or...
To assess cardiomyocyte hypertrophy, serial 5 µm paraffin heart sections were deparaffinized, hydrated, antigen retrieved, and stained with Alexa Fluor 488 WGA (5 µg/mL, Invitrogen) for 1 hour at room temperature (RT)32. Nuclei were counterstained with 4’,6-diamidino-2-phenylindole...
aThis means i could get up an hour later than usual,as schools in China begin before 8 a.m. 这意味着我可能起来晚1小时比通常,学校在中国在上午8点之前开始。[translate] adiveide diveide[translate] aWhy is it no pleasure looking at nature though the curtains 为什么是它没有虽则看自然的乐趣...
For I had a little drink about an hour ago And it went right to my head* Wherever I may roam On land or tea or foam You will always hear me singing this song: Show me the way to "go home. Why don't you*.* Indicate the way to my abodey I'm fatigued and I want to retire...
12 July 2016 Sox transcription in sarcosine utilization is controlled by Sigma54 and SoxR in Bacillus thuringiensis HD73 Qi Peng1, Chunxia Liu1, Bo Wang1,2, Min Yang1, Jianbo Wu1, Jie Zhang1 & Fuping Song1 Sarcosine oxidase catalyzes the oxidative demethylation of sarcosine ...
hour meetings goals Kindle and members PowerPoint and Prime and single-threaded leadership six-pagers and two-pizza teams and S-Team goals Amazon Digital and annual planning process Dive Deep leadership principle and finance team’s tracking of growth and evolution of high bar for input-focused ...