The present invention relates to a novel variant RNA polymerase sigma factor 70 (π) polypeptide, a polynucleotide encoding the same, a microorganism containing the polypeptide, and a method for producing L-threonine by using the microorganism.Ji Sun Lee...
The first sigma factor discovered was the sigma70 (σ70) of the highly studied bacterium Escherichia coli. Its discovery in 1968 was an unexpected result of trying to understand the subunit structure of the RNA polymerase. It was found that the RNA polymerase exists in two forms. A core ...
Sigma(70)家族对生物农药合成的调控及其 应用 潘盛淇,裘娟萍 (浙江工业大学生物与环境工程学院,杭州310014) 摘要:生物农药作为一种安全低毒、环境友好新型农药具有巨大的市场应用和研究价值。Sigma因子作为细菌基因转 录起始的依赖因子,对生物农药合成起着重要的调控作用。阐述了sigma(70)家族的分类、结构及功能,综述了...
(also known as RNA pol sigma70 and rpoD) is a part of a family of sigma factor subunits that are required for specificpromoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factorresponsible for RNA polymerase recognition out of the majority of promoters necessary...
Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulati... sigma E, an RNA polymerase sigma factor of apparent M(r) 28,000, was previously identified by its ability to direct transcription from the...
Helmann The sigma70 family of sigma factors Genome Biol., 4 (2003), p. 203 View in ScopusGoogle Scholar 6 K.S. Murakami, S. Masuda, E.A. Campbell, O. Muzzin, S.A. Darst Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex Science, 296 (2002), pp....
Ling J, Sharma M, Bhagwat AA (2008) The role of RNA polymerase sigma-factor (rpos) in induction of glutamate-dependent acid- resistance of Escherichia albertii under anaerobic conditions. FEMS Microbiol Lett 283:75-82Ling, J., M. Sharma, and A. A. Bhagwat. 2008. Role of RNA polymerase...
coli RNA polymerase only and sigma factor 70 for transcription was prepared. Approximately 0.75 mg/ml of Firefly luciferase and enhanced green fluorescent protein were produced in batch mode. A plasmid was optimized with different regulatory parts to increase the expression. In addition, a new eGFP...
The recognition of promoter region -10 nucleotide sequences in prokaryotes is believed to be mediated by a segment of alpha-helix in a region of RNA polymerase sigma factors called 2.4. Earlier genetic studies implicated Thr-100 in region 2.4 of the Bacillus subtilis sigma factor sigma H in th...
iaessossawoyesf used the upstream regions of four housekeeping genes of OY-M [the protein chain initiation factor IF-3 (infC), 50S ribosomal subunit protein L13 (rplM), 30S ribosomal subunit protein S4 (rpsD), and RNA polymerase sigma70 factor (rpoD) genes] for in vitro transcription as...