RIG-I and MDA5 both contain DExD/H box RNA helicase with two caspase-recruiting domain (CARD)-like sequences and a helicase domain (Yoneyama et al., 2004; 2005). RIG-I, the most important member of RLHs, recognizes cytoplasmic viral RNA such as that of hepatitis V virus and influenza...
Cells were collect- ed at 24 h after transfection, and luciferase activity was measured with a Dual-Luciferase Assay (Promega, Madison, WI, USA) with a Luminoskan Ascent Luminometer (Thermo Scientific, Hanover Park, IL, USA) as described previously [39]. Reporter gene activ- ity was ...
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