第一轮RT-PCR引物‘91为2F、2R,巢式PCR引物为a、b。 5.2R■PCR反应体系及反应条件 反应体系 DEPC H20 10×One Stepbuffer(M92+、 dNTP混合液(各10mmol/L) RNaseInhibitot AMVRNaseXL AMV OptimizedTaq gag Primers: 2F(109m) 2R(109m) Template l× 11.6UL 2|工L 1.6UL 0.4t-tL O.4pL 0.4p-...
Single-round multiplex PCR with species-specific mitochondrial primers of P. falciparum, P. vivax/P. simium and P. malariae/P. brasilianum: Comparison with standard techniques - ScienceDirectWilson Domingues aEmilly Henrique dos Santos a
We developed and evaluated a semi-nested PCR assay for the detection of Babesia aktasi infection in goats based on the sequence of the B. aktasi 18S ribosomal RNA gene. Following in silico screening, the specificity of the primers was assessed using reference DNA samples, including B. ovis,...
Using the amplified DNA as target, different PCR and sequencing strategies were applied with consensus or specific primers for the study of the central genome part of the putative tit adenovirus. The sequence of supposedly one half (13,628 bp) of the genome was determined including eight full ...