RNA在细胞内具有明确的定位。与蛋白质研究领域已建立的成熟蛋白质标记和成像的方法不同,RNA检测与标记的发展还处于起步阶段。RNA标记方法,如基于MS2,RNA适体和pumilioRNA结合序列的RNA标记方法都是基于非共价作用,结合力比较低,这制约了对丰度较低RNA的标记。 基于共价连接的RNA标记为在RNA上只共价连接一个化学小分子...
Product NameGAPDH antibody MF CAS Product NameHuman TNF-alpha ELISA Kit MF CAS Product NameANNEXIN V antibody MF CAS Product NameLC3 antibody MF CAS Product Name(RS)-MCPG MFC10H11NO4 CAS146669-29-6 Product Name(R)-MG132 MFC26H41N3O5 ...
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人ESCC 细胞株ECA109增殖、凋亡、放射敏感性。方法 取ESCC 肿瘤组织与癌旁组织标本各89例份；另取ECA109细胞，并随机分为对照组（Control 组，空白培养基处理）、si NC 组（转染si NC 质粒）、si -SNHG17组（转染si -SNHG17质粒）、si -SNHG17 + inhibitor NC 组（si -SNHG17与inhibitor NC 共转染）...
"891","Gapdh" "892","Gapdhs" "893","Gart" "894","Gas1" "895","Gba" "896","Gcgr" "897","Gdf3" "898","Gdpd2" "899","Gdpd5" "900","Gfap" "901","Gfra1" "902","Gfra2" "903","Gfra3" "904","Gfra4" "905","Gfral" "906","Gfy" "907"...
Primers for the stem cell markers containing VEGF, BMP-6, HGF, KGF, PDGF-α, PDGF-β, C-Myc and the housekeeping gene GAPDH were synthesized commercially (Shengong, Co. Ltd. Shanghai, China). Quantification of all cDNA of stemness marker genes was performed with Bio-Rad MyiQ single ...
The levels of myelination-related gene mRNA transcripts to the contorl GAPDH in individual groups of RSC96 cells were quantified by RT-qPCR. 2.3. In vivo The animal experiment protocols were approved by the Animal Experiment Ethics Committee of Shanghai Ninth People's Hospital affiliated to Shang...
GAPDH expression levels. The PCR primers were as follows:P16, 5’-TAGTCCTTCCTACCCCAATTTCC-3’-(forward), and 5’-TTGGTCCTTAGCCACTCCTTC-3’-(reverse).IL-6, 5’-TACCCCGATTCAGGTGAT-3’-(forward), 5’-TTGAGCAGAAGAGCTGCTACGT-3’-(reverse). AndGAPDH, 5’-AGGTCGGTGTGAACGGATTTG-3’-...
thatBoGAPDHgenes were differentially expressed under stress, which was consistent with the results of qRT-PCR. We cloned and analyzed the differentially expressed geneBoGAPCand found that it was in the down-regulated mode 1 h after self-pollination, and the expression was the highest in the ...
C2C12 myotubes were treated with LPS (1 µg/mL) and the (a) ISE or (b) BuOH fraction of ISE (50, 75, and 100 µg/mL) for 24 h. The mRNA expression of IL-6, IL-1β, iNOS, and COX2 was determined using RT-PCR. GAPDH was used as the internal controls. Bars with a ...