Internationally,UNESCO has called for a banon human germline gene editing. And the "Convention for the Protection of Human Rights and Dignity of the Human Being with regard to the Application of Biology and Medicine" – theOviedo Convention– specifies that "an intervention seeking to modify the ...
The most potent use of the new gene editing technique CRISPR is also the most controversial: tweaking the genomes of human embryos to eliminate genes that cause disease. We don’t allow it now. Should we ever?
Tissue stem cells may be subject to less-stringent epigenetic restrictions than other cells and might therefore be more amenable to deprogramming. We report... J Silva,O Barrandon,J Nichols,... - 《Plos Biology》 被引量: 1464发表: 2008年 Ethics of Human Genome Editing Advances in human ge...
proposing that ethical oversight combined with genetic scrutiny at the embryo stage post-editing can enable responsible use of the technology, ultimately reducing the burden of genetic diseases and enhancing human health, akin to how IVF transformed reproductive medicine despite strong initial opposition....
The real question is not whether gene editing – or smart drugs, or any other emerging technology for that matter – should be allowed or banned, but how it should be regulated. First, prohibition and permission are just two modes of regulation on a scale that includes at least the followin...
As well as an optimized assay and a good sgRNA library, a successful CRISPR screen needs a biologically relevant model to ensure confidence in the resulting hits. Primary cells can be incompatible for pooled library screening with CRISPR, especially those from the human nervous system, those not...
Until recently, unintended off-target changes in the genome caused by classical genetic engineering technologies were seen as a major safety obstacle for using them for human germline modifications. The very recent rapid progress in increasing the precision of genome editing by CRISPR-Cas 9 in order...
Sequencing reads were mapped to the human genome GRCh38 (Ensembl v101) and Ensembl’s reference transcriptome (v101) using a single-pass of STAR with the following arguments changed from their default: seedSearchSartLmax 12, outFilterType BySJout and alignEndsType Local. FeatureCount was used ...
G protein-coupled receptors (GPCRs) represent one of the most important protein superfamilies encoded by the human genome. The members of this protein superfamily (more than 800 entities [1]) have been proven to perform a great variety of biological functions in the organism. Among these, very...
Nearly three decades since its genetic definition, which have seen the completion of the Human Genome Project and the development of 2nd- and 3rd-generation sequencing strategies, a reappraisal of current LS screening strategies, and consideration of how these may evolve, is timely. Furthermore, ...