Shotgun metagenomicsHost–pathogen molecular interactionsSoft-rot EnterobacteriaceaePolymicrobialIn silicoShotgun metagenomic sequencing of bacterial communities in necrotic plant lesions allows insights of host–pathogen molecular interactions. Soft-rot Enterobacteriaceae are significant crop pathogens with a wide ...
High-throughput sequencing technologies and a suite of computational pipelines have been combined into shotgun metagenomics methods that have transformed microbiology. Still, computational approaches to overcome the challenges that affect both assembly-based and mapping-based metagenomic profiling, particularly ...
High-throughput sequencing technologies and a suite of computational pipelines have been combined into shotgun metagenomics methods that have transformed microbiology. Still, computational approaches to overcome the challenges that affect both assembly-based and mapping-based metagenomic profiling, particularly ...
Here, we describe a workflow for DNA recovery, metagenomic shotgun sequencing and in particular, an in silico analysis of bacterial isolates from affected plant tissue. 展开 关键词: Shotgun metagenomics Host–pathogen molecular interactions Soft-rot Enterobacteriaceae Polymicrobial In silico 出版时间: ...
Introduction: Whole Genome Shotgun Sequencing (WGS) of the microbiota is a powerful technique that provides an unbiased look at the relative abundance of the gut microbiome, but the analysis of the large data sets requires a bioinformatician using software executed on high-powered servers. This ...
Shotgun metagenomics by high-throughput sequencing may allow deep and accurate characterization of host-associated total microbiomes, including bacteria, viruses, protists and fungi. However, the analysis of such sequencing data is still... B Fosso,M Santamaria,M D'Antonio,... - 《Bioinformatics...
The input for this analysis is paired end next generation sequencing data from metagenomic samples. The workflow is designed to be modular, so that individual modules can be run depending on the nature of the metagenomics project at hand. More modules will be added as we develop them - this ...
Metagenomics has recently revealed the composition ... NC Verberkmoes,AL Russell,M Shah,... - 《Isme Journal》 被引量: 1116发表: 0年 Colorectal Cancer and the Human Gut Microbiome: Reproducibility with Whole-Genome Shotgun Sequencing Accumulating evidence indicates that the gut microbiota affects ...
Metagenomics Reveals a Core Macrolide Resistome Related to Microbiota in Chronic Respiratory Disease relate them to host and environmental microbiomes using ultra-deep metagenomic shotgun sequencing Methods: Airway specimens from n=85 individuals with and wit... MM Aogáin,KJX Lau,C Zhao,... - 《...
Sequencing and analysis approach To obtain an unbiased picture of microbial diversity and microbial load in wild A. thaliana plants, we took a metagenomic shotgun sequencing approach to analyze entire wild phyllospheres. Because plant genomic DNA was expected to dominate such samples, we initially at...