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To determine whether Orc2-SH stimulates ATP hydrolysis by OCCM via Mcm2-7 binding, we conducted a GST pulldown assay to measure the ability of Orc2-NTD (residues 1–200) (referred to as Orc2N200) binding to MCM-containing complexes. Purified GST-Orc2N200 was immobilized on glutathione re...
Notes: If you’ve never set an alias before, don’t know where to put them, or have no clue what I’m talking about, read my post onTerminal/Bash Command-Line Shortcuts with Aliasesbefore continuing. When copy & pasting, it’s important to keep the spacing. (i.e. for aliases, the...
Indeed, in a prior SELEX study using recombinant GST-YB1, multiple RNAs containing GCACC were enriched (43), consistent with our conclusion.Figure 9. Identification of YB1 as a potential splicing repressor bound to GCACC. (A) RNA-affinity purification was performed to explore RBPs that bind...
Neither the direct or the indirect experiment showed any effect on the expression of the studied CYP, GST or AChE genes; however, a large family-level variation in expression profiles was found in both the direct and the indirect experiments. The large observed variation in expression profiles ...
To prepare an anti-γSyn antibody, we immunized New Zealand white rabbits with GST-conjugated recombinant γSyn that had been expressed in bacteria and purified using GST-Sepharose (GE Healthcare, UK) according to standard procedures. The antiserum was collected and used in the experiments (Supple...
A Report on a Book-in-Progress Keepsake Number 3. GBP 10.001478091034 ABAKNOWICZ, MAGDALENA (ARTIST.); MARLBOROUGH (NEW YORK) Magdalena Abakanowicz, Mutants. January 15 - February 11, 1997. Marlborough. New York, Ny[Exhibition Brochure] USD 75.00...
pay taxes on income from active business operations, property income and capital gains arising from the sale of assets. Taxes other than income taxes such as GST and PST are added to the sales price, collected from customers, and then remitted to the federal and provincial ...
What is the impact of GST on the PPC curve in the short run or long run? What will be the impact on inflation after implementation of a GST? What are the harmful implications of monetary expansion? What are the effects of cryptocurrency on the South African g...
Impact of Demonetization and Goods and Services Tax (GST) on India's Economic Growth: A Critical Review India's economic growth has been affected by the sudden withdrawal of currency notes from the circulation and hasty implementation of GST (Goods and Services Tax) in the economy. Demonetization...