doi:10.1016/S0167-7012(98)00028-1George MulveyRosa VanmaeleMonique MrazekMark CahillGlen D. ArmstrongJournal of Microbiological MethodsMulvey G, Vanmaele R, Mrazek M, Gahill M, Armstrong GD. 1998. Affin- ity purification of Shiga-like toxin I and Shiga-like toxin II. J Microbiol Methods. ...
Shiga-like toxin type II (SLT-II) and Shiga-like toxin type II variant (SLT-IIv) are cytotoxins produced by certain strains of Escherichia coli. Nucleotide sequence analyses had revealed that the structural genes for the A subunit and B subunit of SLT-II or SLT-IIv are arranged in an ope...
Shiga Toxin: Purification, Structure, and Function Shiga toxin is a potent toxin produced by Shigella dysenteriae type 1 strains. The toxin has three biologic activities--cytotoxicity, enterotoxicity, and neurotoxicity--and one known biochemical effect: inhibition of protein synthesis. I... DR Arthur...
Shiga-like toxin typeⅡvariant (SLT-Ⅱe) is composed of one A subunit and five B subunits. 利用重叠延伸PCR法对志贺样毒素 型变异体A亚单位(SLT- eA)基因进行修饰扩增,将第167位编码Glu的密码子突变为Gln的密码子,第170位编码Arg的密码子突变为编码Lys密码子,并用质粒载体pGEX-6P-1在大肠杆菌BL21中...
The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of signifi...
Two differentShiga-like toxinsinvolving Shiga-like toxin 1 and 2 are recognized inE. coliO157:H7 that both are produced via encoding genes in association with prophages. Shiga-like toxins are responsible for bloody diarrheal symptom and hemolytic uremic syndrome. They are structurally consisted of an...
Shigella dysenteriae and Shiga-toxin-producing Escherichia coli (STEC) elaborate the AB holotoxins, Shiga or Shiga-like toxins (Stx). Stx play a major role in the pathogenesis of haemorrhagic colitis and haemolytic uremic syndrome. This review provides an overview of the mechanisms of action of ...
E. coli 0157:H7 were detected in 2 CD and in 1 HUS patient. Fifty-one percent (21/41) HUS patients, 21% (10/47) CD and 5% (1/19) healthy children had serum neutralizing titers of 鈮 1:4 to shigatoxin (p<0.0005). These date show that HUS is associated with SLT-producing E...
Fifty-one percent (21/41) HUS patients, 21% (10/47) CD and 5% (1/19) healthy children had serum neutralizing titers of ≥ 1:4 to shigatoxin (p<0.0005). These date show that HUS is associated with SLT-producing E. coli and suggest that the high frequency of HUS in Argentina is ...
GEMSKI, and A. A. LINDBERG, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA, and Karolinska Institute, S-141 86 Huddinge, SwedenShow moreShow less Choose an option to locate/access this article: Check if you have access through your login credentials or your ...