Devices: Beckman system (p / ace system 2050) using geol software (version 7.11 U); Capillary: Silice (TSPO 50375), 50 [mu] m x 97 cm; Buffer: 2M formic acid; Coated solution, 5% p / v hexadimethrene bromide and 2% v / v ethylene glycol aqueous solution; Detector: UV (200 nm)...
4 filter paper under vacuum, lyophilized and used for further characterization. 3.3. Purification by Gel Filtration Chromatography Gel filtration chromatography was performed to purify CIIA, CIIP and GII. In brief, samples (100 mg) were dissolved in 10 mL of a running buffer (20 mM sodium ...