shRNA:小发卡或短发卡RNA(a small hairpin RNA or short hairpin RNA,shRNA)是一段具有紧密发卡环(tight hairpin turn)的RNA序列,常被用于RNA干扰沉默靶基因的表达。利用载体把shRNA导入细胞,载体中的U6启动子确保shRNA总是表达;shRNA的发卡结构可被细胞机制切割成siRNA,然后siRNA结合到RNA诱导沉默复合物上(RNA-...
片段类型shRNA 片段种属小鼠 原核抗性Amp 筛选标记Neo/G418 荧光标记红色 感受态DH5a 培养温度37℃ 储存温度-20℃ 产品声明在购买产品前,请务必仔细阅读下方的产品声明,如购买Solarbio质粒系列产品,表示你完全接受本声明全部条款。1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;2、我们...
pU6-TWIST1(human)-lncRNA-shRNA1-SV40-Neo 市场价:¥1184.00 商城价:¥948.00 100% 好评率 4 累计销量 满200包邮:消费满200元包邮商品品牌: 索莱宝 包装规格: 1株商品运费: 运费10元 商品数量: (库存5)立即购买 加入购物车 咨询 收藏 举报...
ANTISENSE RNABackground: Long noncoding RNAs (lncRNAs) play vital regulatory functions in non-small cell lung cancer (NSCLC). Cisplatin (DDP) resistance has significantly decreased the effectiveness of DDP-based chemotherapy in NSCLC patients. This study aimed to investigate the effects of SH3PXD2A...
Therefore, we conclude that the upregulation of lncRNA SH3PXD2A-AS1 may contribute to the pathogenesis of preeclampsia through prohibiting trophoblast invasion during placentation.doi:10.1038/s41419-020-02796-0Qian ChenSijia JiangHaihua LiuYue Gao...
OPEN Cell Death and Differentiation (2018) 25, 307–318 Official journal of the Cell Death Differentiation Association www.nature.com/cdd A large shRNA library approach identifies lncRNA Ntep as an essential regulator of cell proliferation Julia Beermann1, Dominique Kirste1, Katharina Iwanov1, ...
中lncRNA-miR210HG,SH3BGRL3表达水平高于癌旁组织(P<0.05).lncRNA-miR210HG阳性表达者Ⅲ期占比,淋巴结转移占比高于阴性表达者;SH3BGRL3阳性表达者Ⅲ期占比,淋巴结转移占比,低中分化占比高于阴性表达者(P<0.05).lncRNA-miR210HG,SH3BGRL3阳性组总生存率均低于阴性组(P<0.05).结论lncRNA-miR210HG,SH3...
Expression of the two lncRNA candidates in SH-SY5Y cells was the maximum after incubation under the OGD condition for 8 h. The knockdown efficiency was more than 60% for four of the six siRNAs, and knockdown of NR_120420 increased the cell viability and decreased the percentage of TUNEL-...
CAS号: 英文名: pLV2-U6-TUG1(rat)-LncRNA-shRNA1-EGFP-Puro 英文别名: 中文名: PLV2-U6-TUG1(RAT)-LNCRNA-SHRNA1-EGFP-PURO 中文别名: PLV2-U6-TUG1(RAT)-LNCRNA-SHRNA1-EGFP-PURO CBNumber: CB713301382 分子式: 分子量: 0 MOL File: Mol file ...
与RNA测序相比,芯片检测的准确性受低丰度转录本的影响更小,更适合检测整体表达丰度低的lncRNA。由于提高测序深度会增加加工不完全RNA的检出机率,提高测序深度可能引起可靠性的下降。lncRNA不具有蛋白的翻译起止位置,RNA测序对lncRNA转录本鉴定的可靠性要低于mRNA。而芯