When we tested time-sequential samples from four patients with SFTS, antibody to SFTSV-NP were detectable not only during the recovery phase (days 10–622) but also during the acute phase (days 4–7) of the disease using both of a double-antigen ELISA and IC assay. SFTSV-RNA was ...
首先以真核蛋白表达载体pAB为基础构建SFTSV Gn-Fc重组质粒,并通过293F细胞瞬时转染高效表达蛋白系统表达SFTSV Gn-Fc蛋白并提取纯化,之后用Gn-Fc作为抗原免疫小鼠,利用杂交瘤细胞融合技术制备SFTSV Gn单克隆抗体(monoclonal Antibody,mAb).然后用前期包装成功的以VSV为基础载体的SFTSV M假病毒筛选出具有特异性的单克隆...
SFTSV NP rabbit polyclonal antibody was customized by Atagenix Biological Technology Co., Ltd. (Wuhan, China). Cell viability assay CAGGTG-3′ (upstream) and 5′-ATCTTACCTCCGCCA TGTTG-3′ (downstream). The qRT-PCR primers used for GAPDH were 5′-TGATGA...
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11001; RRID:AB_2534069 Polyclonal serum to SFTSV NP (Anti-NP) Wenhui Li (National Institute of Biological Sciences) N/A Monoclonal anti-HRTV NP (2AG8) Amanda E. Calvert (U.S. ...
Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection. Braz. J. Infect. Dis. 2022, 26, 102386. [Google Scholar] [CrossRef] Shimada, S.; Posadas-Herrera, G.; Aoki, K.; Morita, K.; Hayasaka, D. Therapeutic ...