siRNAPAMAMIntroduction: Excessive circulating sFlt1 plays a major role in the pathogenesis of preeclampsia (PE). Using RNAi to silence sFlt1 may be a therapy for treating PE. Because of the rapid degradation of siRNA, gene therapy in vivo remains limited. Poly-amidoamine (PAMAM) has been ...
关键词:VEGF;内皮细胞损伤;siRNA华中科技大学博士学位论文3第三部分缺氧滋养细胞通过分泌sFlt-1下调VEGF途径导致内皮细胞损伤的研究目的:1.研究缺氧对滋养细胞及内皮细胞中sFlt-1表达的影响,探讨子痫前期sFlt-1表达异常的原因。2.研究缺氧滋养细胞对内皮细胞损伤的影响及其sFlt-1/VEGF损伤途径,从细胞水平深入探讨子痫...
siRNA experiments targeting each variant confirmed that three of the four variants contribute significantly to total sFlt1 expression by cytotrophoblasts in vitro. These findingsprovide evidence that human placenta expresses a family of sFlt1 splice variants, at least three of which are expressed as ...
Silencing of TFAP2A using Trilencer siRNA upregulated sFLT1 expression only in NT-DSCs but suppressed the expression of decidualization markers PRL, IGFBP1 and their regulator FOXO1 in cells from both groups. Collectively, our observations suggest that TFAP2A acts as a repressor of sFLT1 and ...