JCV strains and plasmid constructs The wild type Mad1 strain of JCV was linearized by BamH1 digestion, and cloned into the pBlueScript KS (+) vector previously [14]. To mutate the second 98 bp repeated region on JCV promoter, the pBlue-Mad1-WT construct was digested with Avr II restric...
Early in vitro studies showed that ASF/SF2 is able to commit pre-mRNA substrates to splicing, indicating that it interacts with the pre-mRNA at the earliest step in the splicing reaction (Fu, 1993). The ability of ASF/SF2 to cooperate with U1 snRNP to recognize 5′ splice sites is also...