最后找到了 seurat 的官方镜像 satijalab/seurat。 使用R 解释器验证 library(Seurat) library(SeuratDisk) 一了百了,折腾了一天,那个男人终于到手。 附硬核的 Dockerfile,很简洁。 # Dockerfile for Seurat 4.3.0FROMrocker/r-ver:4.2.0# Set global R optionsRUNecho"options(repos = 'https://cloud.r-pro...
─ building ‘SeuratDisk_0.0.0.9021.tar.gz’ 将程序包安装入‘/home/xxx/R/x86_64-pc-linux-gnu-library/4.3’ (因为‘lib’没有被指定) ERROR: dependency ‘hdf5r’ is not available for package ‘SeuratDisk’ * removing ‘/home/xxx/R/x86_64-pc-linux-gnu-library/4.3/SeuratDisk’ Warning m...
# 3.使用library(Seurat)library(SeuratDisk)setwd('/home/name/path_to_your_files/')# 转换 .h5ad 文件为 .h5seurat 文件Convert("./data/Global_lognormalised.h5ad",assay="RNA",dest="h5seurat",overwrite=TRUE)# 读取 .h5seurat 文件为 Seurat 对象seurat_object<-LoadH5Seurat("./data/Global_logn...
RStudio 载入SeuratDisk报错 在终端R安装了SeuratDisk,并且可以载入,但是在网页RStudio则载入报错: > library(SeuratDisk) 错误: package or namespace load failed for ‘SeuratDisk’ in dyn.load(file, DLLpath = DLLpath, ...): 无法载入共享目标对象‘.../Rlibrary/hdf5r/libs/hdf5r.so’:: libhdf5_...
SeuratDisk v0.0.0.9015 The h5Seurat file format is specifically designed for the storage and analysis of multi-modal single-cell and spatially-resolved expression experiments, for example, from CITE-seq or 10X Visium technologies. It holds all molecular information and associated metadata, including ...
R包hdf5r安装失败 SeuratDisk 安装失败 R包hdf5r安装失败Found hdf5 with version: 1.8.12configure: error: The version of hdf5 installed on your system is not sufficient. Please ensure that at least version 1.8.13 is installedERROR: configuration failed for package ‘hdf5r’...
My code is as follows install.packages("remotes") remotes::install_github("mojaveazure/seurat-disk") error is as follows 停止执行 ERROR: lazy loading failed for package 'SeuratDisk' removing 'C:/Users/user/Documents/R/win-library/4.0/SeuratD...
devtools::install_github("mojaveazure/seurat-disk")# 然后我换了本地安装还是保错install.packages('D:/IEDown/seurat-disk-master.zip', repos = NULL)以下是错误信息:Error: Failed to install 'unknown package' from GitHub: Timeout was reached: [api.github.com] Connection timeout after 10003 ms我...
SeuratDisk is not currently available on CRAN. You can install it fromGitHubwith: if(!requireNamespace("remotes",quietly=TRUE)){install.packages("remotes")}remotes::install_github("mojaveazure/seurat-disk") Dependencies SeuratDisk depends on the following non-standard packages: PackageCRAN...
─ building ‘SeuratDisk_0.0.0.9009.tar.gz’ Warning: invalid uid value replaced by that for user 'nobody' Warning: invalid gid value replaced by that for user 'nobody' installing source package ‘SeuratDisk’ ... ** using staged installation ** R ** byte-compile and prepare package for...