In addition, another parameter that was taken into account for protein selec- tion was the MW; proteins with MW not conforming with those indicated by the MW marker standard were not considered. Two-dimensional gel electrophoresis of serum proteins. The 2-DE coupled to MS analysis con- ...
Isoelectric points and molecular weight were estimated by parallel electrophoresis with a mixture of pI and Mr protein standards (Bio-Rad). Qualitative and quantitative analysis of protein patterns were conducted by using PDQuest. Master gels were created from four replicated gels from each sheep at...
brucellosisMSDairy cows2-DEIn order to provide an evidence of brucellosis for diagnosis and prevention, 2D-electrophoresis and SDS-PAGE were applied to detect protein expression difference in plasma between healthy dairy cows and dairy cows suffered from Brucellosis. The results showed that 11 ...
Two-dimensional gel electrophoresis is limited in terms of its sensitivity in identifying proteins with concentrations in the pg/ml range, especially if they may be masked by abundant proteins present in the biofluid of interest. However, methods such as stable-isotope protein tagging or subtractive...
0 image analysis software was used to analyze the differences in two-dimensional gel electrophoresis. Matrix assisted laser desorption ionization. Time of flight mass spectrometry was used for serum proteome. RESULTS There were thirty-five protein spots with obvious differences,and six proteins were ...
Differential protein analysis was performed using 2-dimensional differential in-gel electrophoresis (2D-DIGE). Results Among these protein spots, we found that 16 protein spots were differently expressed between the two mixtures; 8 of these were up-regulated and 8 were down-regulated in cancer. ...
A total of 13 search engines was used for glycopeptide identification: IQ-GPA v2.530, Protein Prospector v5.20.2331, glyXtoolMS v0.1.432, Byonic v2.16.1633, Sugar Qb34, Glycopeptide Search v2.0alpha35, GlycopeptideGraphMS v1.0/Byonic36, GlycoPAT v2.037, GPQuest v2.038, Mascot v2.5.163 or...
All DNA–protein complexes were resolved by electrophoresis on 5% native polyacrylamide and transferred to Immobilon-Ny+ membranes (Millipore, Billerica, MA, USA). Quantitative real-time polymerase chain reaction The total RNA of RPMI 8226 cells cocultured with 100 μg/mL SD MVs or non-SD MVs ...
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As an ana- lytical technique, two-dimensional difference gel electrophoresis (2D-DIGE) provides an insight into intact and fragmented protein species and also provides information regarding post-translational modifications. 2D-DIGE incorporates the use of fluorescent molecules (CyDyes), or fluors, ...