PROBLEM TO BE SOLVED: To provide Serum Bactericidal Activity (SBA) assays for Gram negative bacteria, in particular N. meningitidis.ISABEL DE VLEESCHAUWERド フレースハウウェル,イザベルNATHALIE DURANTデュラント,ナタリーPOOLMAN JANプールマン,ジャン...
The reasons are complex but ultimately have to do with the effects of potential disparities between in vitro antibody functional assay conditions and in vivo host defenses” [94]. The critical role of SBA as a surrogate for efficacy, through the measure of complement mediated bactericidal ...
Objective To develop a serum bactericidal assay(SBA)in vitro for determination of bactericidal activity of serum anti-Haemophilus influenzae type b(Hib)polyribosylribitol phosphate(PRP)antibody.Methods A SBA method was developed by screening of complement and index bacterial strain,and verified for stabi...
Ex-vivo assessment of meningococcal resistance to natural immunity: serum bactericidal assays versus whole- blood assay Zahra Eslami Nejad,Nikoo Nik Nafs,Nosratollah Saeed Adeli Keywords: Neisseria meningitidis , meningococci , SIC , SBC , SBA , WBA Full-Text Cite this paper Add to My Lib ...
The "gold standard" assay for measuring serologic protection against Neisseria meningitidis group B (MenB) is the serum bactericidal antibody (SBA) assay. Of vital importance to the outcome of the SBA assay is the choice of the target strain(s), which is often chosen on the basis of phenoty...
assay do not consistently distinguish between bactericidal and nonbactericidal anticapsular antibodies.243,244,254,257–259Therefore, serologic assessments of meningococcal vaccine immunogenicity rely on measurements of complement-mediated SBA. In this assay, the test serum is heated to inactivate endogeno...
Before immunization, human complement-mediated serum bactericidal titers 11 (SBA) ≥1:4 were present in 19, 11 and 8% of subjects, respectively, against group B 12 strains H44/76, NZ98/254 and S3032, which increased to 97, 22 and 36%, respectively, 13 1 month after dose 3 (P<0.01...
1) Serum bactericidal assay 血清杀菌力试验2) Bactericidal test 杀菌试验 1. Some method which medium making and chemical drug bactericidal test are newly improved in the paper. 本文在革兰氏染色中采用正丙醇代替95%酒精脱色,解决了因脱色时间过长和老龄菌体染色 假阴性问题,并对培养基的制作、化学...
MENINGITIDIS SPEZIFISCHE ANTISERENThe present invention relates to the field of Serum Bactericidal Activity (SBA) assays for Gram negative bacteria, in particular N. meningitidis. The SBA assay is the most important method for measuring functional activity of serum antibodies against meningococcus. In...
Gram-negative bacteria, in particular, the invention relates to the field Serum bactericidal activity against N. meningitidis (SBA) assay. SBA assay is the most important method for measuring the functional activity of the serum antibodies against Neisseria meningitidis. To determine population subject ...