An antibody raised against a 12 amino acid peptide corresponding to the C-terminal sequence of the SERCA-2b Ca2+,Mg2+- ATPase precipitated Ca2+,Mg2+-ATPase activity from pancreatic rough ER. Thapsigargin and vanadate inhibited the activity with the same concentration-dependence as ...
DDM, n-dodecyl-β-D-maltoside; pAb, polyclonal antibody; SERCA2b, sarco(endo)plasmic reticulum Ca2+-ATPase 2b; TER, trans-2-enoyl-CoA reductase. We assessed whether endogenous TER bound to endogenous SERCA2b by coimmunoprecipitation analysis. The Triton X-100 extracts of HEK293 cells were ...
To further validate the interaction between PERP and SERCA2, HaloTag and Halo-PERP were expressed in Mel202 cells and SERCA2 interacting proteins were isolated by immunoprecipitation (IP). The specific isolation of Halo-PERP was confirmed by Western blot using a HaloTag antibody (Fig.1c). Toge...
Four regions of the brain were analyzed with three immunological reagents: a monoclonal antibody that recognizes both forms of SERCA2, and two antisera which are specific for the two alternative forms, SERCA2b or SERCA2a. Cerebellar Purkinje cells express predominantly SERCA2b but also low levels ...
An antibody raised against a 12 amino acid peptide corresponding to the C-terminal sequence of the SERCA-2b Ca2+,Mg2+- ATPase precipitated Ca2+,Mg2+-ATPase activity from pancreatic rough ER. Thapsigargin and vanadate inhibited the activity with the same concentration-dependence as for native ER ...