An antibody raised against a 12 amino acid peptide corresponding to the C-terminal sequence of the SERCA-2b Ca2+,Mg2+- ATPase precipitated Ca2+,Mg2+-ATPase activity from pancreatic rough ER. Thapsigargin and vanadate inhibited the activity with the same concentration-dependence as for native ER ...
As the peptide identified by mass spectrometry could not distinguish between the three isoforms, the specific interaction between PERP and SERCA2b was confirmed after HaloTag pull-down by Western blot using the isoform-specific antibody (Fig. 1b). Fig. 1: PERP interacts with SERCA2b at ER–PM...