Genome editing technologies, including CRISPR-Cas9– mediated editing events, are rapidly becoming accessible to a majority of biological science researchers, and are poised to revolutionize all fields of biology and health care. Thermo Fisher Scientific offers all the tools necessary for a genome editi...
‹Next-Generation Sequencing Education (for Illumina Platforms) DNA Sequencing Technologies–History and Overview Next-Generation Sequencing Illumina Workflow–4 Key Steps Preparation of DNA Sequencing Libraries for Illumina Systems—6 K...
At the same time, there has been growing use of nanopore sequencing for pathogen genomic surveillance, facilitated by the small and portable MinION sequencing device (Oxford Nanopore Technologies). The MinION can be deployed in low resource settings, requires no maintenance, and permits real-time da...
Experimental technologies, such as targeted genotyping coupled with scRNA-seq, have been developed to enable the study of expressional and somatic variations together at single-cell level [3,4,5]. However, the single cells have limited amounts of biological materials, a small number of detectable...
Next-generation sequencing (NGS) technologies have drastically reduced the cost of sequencing and placed a wide range of genomic analyses within the capabilities of many laboratories1,2,3,4. Multiple NGS technologies and platforms coexist and each offers unique advantages and disadvantages in sequencing...
Next-generation sequencing (NGS) has advanced the application of high-throughput sequencing technologies in genetic and genomic variation analysis. Due to the large dynamic range of expression levels, RNA-seq is more prone to detect transcripts with low expression. It is clear that genes with no ...
the genome sequencing was carried out using 10× Genomics linked read and Nanopore long-read technologies. The draft genome assembly ofT. cordifoliawas comprised of 1.01 Gbp, which is the genome sequenced from the plant family Menispermaceae.We also performed the genome size estimation forT. cordi...
). No significant variation in TPM counts between the healthy and infected samples per gene were observed. However, based on the Kruskal-Wallis Rank Sum Test, the TPM count ratio of GAPC2 to ACT2 was significantly higher compared to the TPM count ratio in 2020 (p...
the use of Field Effect Transistors (FETs) is popular due to the great potential for miniaturization, fast responses, parallel sensing structures, and, most significantly, seamless integration with Complementary Metal Oxide Semiconductor (CMOS) processes. Using VLSI technologies such as CMOS processes, ...
Evolutions of DNA sequencing technology First generation sequencing technologies The double helix structure of DNA proposed by Watson and Crick (1953) was the milestone Figure 1.1 NGS platforms as a tool for the plant genomic research. The phenotype of an organism is a manifestation of controlled ...