Genome editing technologies, including CRISPR-Cas9– mediated editing events, are rapidly becoming accessible to a majority of biological science researchers, and are poised to revolutionize all fields of biology and health care. Thermo Fisher Scientific offers all the tools necessary for a genome editi...
At the same time, there has been growing use of nanopore sequencing for pathogen genomic surveillance, facilitated by the small and portable MinION sequencing device (Oxford Nanopore Technologies). The MinION can be deployed in low resource settings, requires no maintenance, and permits real-time da...
Experimental technologies, such as targeted genotyping coupled with scRNA-seq, have been developed to enable the study of expressional and somatic variations together at single-cell level [3,4,5]. However, the single cells have limited amounts of biological materials, a small number of detectable...
Next-generation sequencing (NGS) technologies have drastically reduced the cost of sequencing and placed a wide range of genomic analyses within the capabilities of many laboratories1,2,3,4. Multiple NGS technologies and platforms coexist and each offers unique advantages and disadvantages in sequencing...
the genome sequencing was carried out using 10× Genomics linked read and Nanopore long-read technologies. The draft genome assembly ofT. cordifoliawas comprised of 1.01 Gbp, which is the genome sequenced from the plant family Menispermaceae.We also performed the genome size estimation forT. cordi...
To ensure high-quality DNA was used for sequencing, the purity was measured with a Qubit 2.0 Fluorometer Life Technologies NY (USA). DNA concentration was then normalised to 20 ng/µL. The DNA had a minimum 260/280 ratio of 1.8. 4.2. GBS Library Construction and Sequencing The DNA were...
We will be covering some of the second-generation sequencing (SGS) or next-generation sequencing (NGS) methods, which are for short-read sequencing, and the third-generation sequencing for long-read sequencing. Table 1. Limitations of short-read sequencing technologies. Table 2. Limitations of ...
it remains challenging to accurately identify complex integrated genome structure upon HPV integration using targeted-capture NGS. Third-generation sequencing technologies, such as nanopore sequencing and PacBio sequencing, enable us to elucidate the complex structural aspects of genomes, due to their long...
Next-generation sequencing (NGS) has advanced the application of high-throughput sequencing technologies in genetic and genomic variation analysis. Due to the large dynamic range of expression levels, RNA-seq is more prone to detect transcripts with low expression. It is clear that genes with no ...
Evolutions of DNA sequencing technology First generation sequencing technologies The double helix structure of DNA proposed by Watson and Crick (1953) was the milestone Figure 1.1 NGS platforms as a tool for the plant genomic research. The phenotype of an organism is a manifestation of controlled ...