For the scATAC-seq data, we used this integrated PBMC reference to predict cell types. A gene activity matrix was first estimated, and label transfer was performed by assigning query cells based on the local ne
The SIRV-Set 4 module is a set of synthetic transcripts that provide processing controls for determining isoform complexity based on length, alternative splicing events, and abundance. The SIRV-Set 4 module includes long SIRV transcripts, SIRV isoforms, and the External RNA Control Consortium tran...
Erectile dysfunction (ED) is a common and difficult to treat disease, and has a high incidence rate worldwide. As a marker of vascular disease, ED usually occurs in cardiovascular disease, 2–5 years prior to cardiovascular disease events. The extra
Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with a five-year survival rate of approximately 11%1. With increasing incidence, it is projected to become the second leading cause of cancer-related deaths in the United States by 20302. Current treatment options are limited. Onl...
6E). Takara® and G&T processed cells also captured the most splice junctions, suggesting higher ability to study alternative splicing events between cells (Fig. 5A). Discussion This study is a performance evaluation of four different plate-based scRNA-seq protocols; NEBNext® Single Cell/ Low...
Studies have shown that the evolution of GST, ABC transporter, and CYP450 gene families in insects has been shaped by a combination of gene duplication, divergence, and loss events, leading to the diversification of their functions and the adaptation of insects to their diets and toxic ...
This method is applicable for the comprehensive analysis of methylation site detection in the epitranscriptome, which will be useful for understanding these methylation events and their mechanisms, ushering in a new era in RNA biology. Methods Materials Reagents and solvents were purchased from standard...
In theory, the frequencies of di-, tri-, tetra-, penta-, and hexanucleotide repeats should progressively decrease, based on the relative probability of replication slippage events. However, trinucleotide repeat units were predominant, followed by tetra-, di-, hexa-, and pentanucleotide repeat ...