For the scATAC-seq data, we used this integrated PBMC reference to predict cell types. A gene activity matrix was first estimated, and label transfer was performed by assigning query cells based on the local neighborhood around each anchor in the integrated reference, with the highest scoring cel...
Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with a five-year survival rate of approximately 11%1. With increasing incidence, it is projected to become the second leading cause of cancer-related deaths in the United States by 20302. Current treatment options are limited. Onl...
Erectile dysfunction (ED) is a common and difficult to treat disease, and has a high incidence rate worldwide. As a marker of vascular disease, ED usually occurs in cardiovascular disease, 2–5 years prior to cardiovascular disease events. The extra
6E). Takara® and G&T processed cells also captured the most splice junctions, suggesting higher ability to study alternative splicing events between cells (Fig. 5A). Discussion This study is a performance evaluation of four different plate-based scRNA-seq protocols; NEBNext® Single Cell/ Low...
In theory, the frequencies of di-, tri-, tetra-, penta-, and hexanucleotide repeats should progressively decrease, based on the relative probability of replication slippage events. However, trinucleotide repeat units were predominant, followed by tetra-, di-, hexa-, and pentanucleotide repeat ...
Studies have shown that the evolution of GST, ABC transporter, and CYP450 gene families in insects has been shaped by a combination of gene duplication, divergence, and loss events, leading to the diversification of their functions and the adaptation of insects to their diets and toxic ...
(4) fusions between adjacent genes were rejected as read-through transcript events, with a minimum distance of 100 kb; (5) finally, we considered a read to support a fusion if it mapped to both sides of breakpoint by at least a minimum fusion anchor length of 25 bp, or generally about...
(d) Sanger sequencing confirmed the splicing events predicted by the Iso-Seq data. total of 2,432 variants (2,357 SNVs and 75 indels) in HX1 were documented in ClinVar24, including 20 variants that were classified as 'pathogenic'. However, a simple allele frequency filter with manual ...
This method is applicable for the comprehensive analysis of methylation site detection in the epitranscriptome, which will be useful for understanding these methylation events and their mechanisms, ushering in a new era in RNA biology. Methods Materials Reagents and solvents were purchased from standard...
and potentially supplants the normal TCR repertoire. Each bar in this 3-D chart represents the sum of all of the TCR sequences that bear the same V-gene and J-gene segment within the patient sample. A veterinarian can use this chart to quickly scan the landscape of the TCR repertoire for...