The present invention provides specific double-stranded DNA/RNA binding compounds having a polymeric structure, which are in fact, triplex forming molecules capable of binding tightly and specifically to predetermined sequences in the major groove of double stranded nucleic acid molecules; as well as ...
PNA was originally designed as a major groove "triple helix binder", but surprisingly subsequent experiments showed that sequence-specific binding to double-stranded DNA took place to homopurine tracts via strand displacement upon formation of an internal PNA 2 /DNA triplex鈥攁 P-loop....
Sequence specific targeting of double-stranded DNA has been a long sought goal of anticancer drug design, because this could open the door to design of gene-targeted drugs. Double-stranded DNA may be recognized sequence specifically either from the minor, or, with greater specificity, from the ...
We report here a method based on the α-hemolysin (α-HL) nanopore, working in conjunction to unmodified citrate anion-coated gold nanoparticles (AuNPs), to detect nanomolar concentrations of short single-stranded DNA sequences (ssDNA). The core idea was to use charge neutral peptide nucleic ...
Sequence-specific pausing occurs during DNA synthesis catalyzed by the bacteriophage T4 DNA polymerase holoenzyme in the presence of the T4 helix destabilizing protein (gene 32 protein). Two of the six strongest pause sites on a double-stranded bacteriophage fd DNA template are in regions where hai...
The DNA targets studied are single-stranded (s-s) DNA, double-stranded (d-s) DNA, d-s DNA with G-G, G-A and G-T mismatches, d-s DNA with... AF Candia,JP Kovalik,CV Wright - 《Nucleic Acids Research》 被引量: 25发表: 1993年 Site-specific stabilization of DNA by a tethered ...
Sequence-specific single-stranded Ku binding occurred rapidly (t1/2 on = 2.0 min) and was exceptionally stable, with an off rate of t1/2= 68 min. While Ku70 cross-linked to the upper strand of NRE1 when Ku was bound to double-stranded and single-stranded DNAs, the Ku80 subunit only...
Presents a study which demonstrated that the interaction between the cellular sequence-specific single-stranded DNA binding protein Pur... and the HIV type... Gallia,Gary,L.,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 59发表: 1999年 Pro...
This affinity matches that of the natural DNA binding domain and is severalfold higher than the affinity of a homologous bovine E2 C-terminal domain (BPV-1) for the same DNA. These antibodies discriminate effectively among a number of double- and single-stranded synthetic DNAs with factors ...
In yet another embodiment a double-stranded DNA fragment is provided which comprises a p53-specific-DNA binding site, wherein the fragment comprises more than one monomer repeat of the sequence 5′-RRRCWWGYYY-3′ (SEQ ID NO: 3) and wherein the fragment is covalently attached to an insolubl...