Here the authors present Specific Terminal Mediated Polymerase Chain Reaction (STEM-PCR) as a generic and accessible approach, and demonstrate proof-of-principle cancer biomarker detection.doi:10.1038/s41467-023-36884-4Gaolian Xugrid.16821.3c0000 0004 0368 8293School of Biomedical Engineering/Med-X ...
but are clearly sequence-dependent: although it is never translated into protein, changing a sequence in the array will reduce slippage. They could be said to be ‘selfishly’ using the mechanism of crossover to replicate, as although their replication is extremely limited and elimination as well...
A synthetic peptide with a deletion of three amino acids at the C terminus of LL-37 (henceforth known as LL-34 parent) retains both its antibacterial activity against group A Streptococcus (GAS) and its ability to promote an inflammatory response in response to U1 dsRNA (30). Since LL-34...
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We present programmable addition via site-specific targeting elements (PASTE), which uses
1c), to encode the two amino acids at the C-terminus of its CDR H3 (Supplementary Fig. 6b). Both COV107-23 and COVD21-C8 bind strongly to the SARS-CoV-2 RBD, with dissociation constants (KD) of 1 nM and 4 nM, respectively (Fig. 3a). However, when their light chains are...
(s) are present in this region, while carboxypeptidases are involved in processing the C-terminus of the CLV3. Recently, Tamakiet al.showed that an endosome-localized SOL1 carboxypeptidase is responsible for removing the C-terminal Arg residue from the CLE19 proprotein [54]. Since intact Ler...
For animal miRNAs, where target selection is strongly based on the seed sequence, the shift at the 5' terminus can clearly influence silencing function (Fig. 1C); however, the potential effect of changes in the length or the sequence of the 3' end could be more problematic to validate. ...
194 of the shared genes are present in a 212 kb direct repeat near the replication terminus (see below). Of the remaining 173 PXO99-specific genes, 29 (including 18 tranposases) are missing from MAFF because they span breakpoints; i.e., a rearrangement, insertion, or deletion in MAFF ...
Proteins destined for the vacuole, for example, have targeting signal peptides found at the N-terminus, at the C-terminus, and at a surface location in mature, folded proteins. Signal peptides also serve as ligands for some receptors. These characteristics of signal proteins can be used to ...
For example, certain modifications to the 3'-terminus can be employed to reduce the susceptibility of the oligonucleotide to nuclease degradation. Moieties which may be appended to the 3'- or 5'-termini include a substituted or unsubstituted amino group, polyethylene glycol, polylysine, acridine,...