Tag Archive You are currently browsing the tag archive for the ‘nilsequences’ tag.Higher uniformity of arithmetic functions in short intervals II. Almost all intervals 11 November, 2024 in math.NT, paper | Tags: Joni Teravainen, Kaisa Matomaki, Maksym Radziwill, multiplicative functions, nil...
Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus l... P Ledent...
Here, we have used our cell free antigen processing system to examine epitope hierarchy under two different conditions; a) among two different protein antigens whose immunodominant epitopes are known, and b) in single recombinant proteins that included spacer/affinity tag sequences for ease of ...
The His6-MBP-N10 tag was removed by the action of TEV protease (TEV: protein = 1:25 v/v) for 1 h at 30 °C. Ni-NTA beads (ThermoFisher Scientific; Cat# 88223) were used to separate the tag from the proteins. The cleaved proteins were diluted in 25 mM Tris HCl ...
Node height (x,y are upper left corner): Height of Node Is the node a HTML block element(Boolean):Referhttps://developer.mozilla.org/en-US/docs/Web/HTML/Block-level_elements. A node is Block Element if it's an Element Node and its tag is one of the following: { "address", "arti...
The typical (His)6tag, present at the C- or N-terminus of a protein which is meant to be purified, has been successfully used for decades. Consecutive histidines are the common denominator for both His-tags used in molecular biology and for quite remote biological phenomena – polyhistidine ...
pEX-N-His PrecisionShuttle™ bacterial vector for inducible expression of a protein with a cleavable N-terminal 6xHis tag. Sequence Author: OriGene Open in SnapGene Try SnapGene for Free Download Plasmid|Download SnapGene Viewer Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets...
We have used a PCR-based approach for the genetical and physical mapping of 34 transcripts isolated from a porcine small intestine cDNA library. All but one gene were regionally localized by using a somatic pig-rodent cell hybrid panel, and 12 genes were mapped by linkage analysis of single-...
random sentence ['tom is finishing his work', 'tom sedang menyelesaikan pekerjaannya'] Input : 3551 Output : 4253 Encoder( (embedding): Embedding(3551, 256) (gru): GRU(256, 512) ) Decoder( (embedding): Embedding(4253, 256)
Bottom, cleavage of His-tag-T4L-XXXXX-3hbtmV2 constructs. Lane 1 of each gel is prestained plus protein ladder. Supplementary Figure 7 Cleavage of other water-soluble and membrane proteins. His-tag-GSHHW-T4L (water-soluble protein; left) lane 1, t = 0; lane 2, cleavage at 18 h. ...