the N terminus of the peptide chain of ##STR3## and conjugate between the same and a carrier protein, as well as hydridoma and monoclonal antibody derived by the use of the polypeptide or the conjugate, and a method of detecting and of purifying human gamma- interferon using the ...
Proteins also exhibit high variability in length, which in human ranges over three orders of magnitudes (from less than 20 AAs for peptide hormones to tens of thousands of AAs in some structural proteins). Such a wide range of protein sequence lengths is prevalent in all domains of life, ...
Peptides are important molecules with diverse biological functions and biomedical uses. To date, there does not exist a single, searchable archive for peptide sequences or associated biological data. Rather, peptide sequences still have to be mined from
The rational drug design against disease-mediating proteins is often based on the 3D structure; however, the flexible structure of IDRs hinders the use of such structure-based design methods. Here, we developed a rational design method to obtain a peptide that can bind an IDR using only ...
(1) a signal peptide; (2) two putative N-glycosylation sites; (3) a single Ig-like fold in the extracellular domain marked by two cysteine residues; and (4) a highly conserved 19 amino acid transmembrane domain containing a positively charged arginine residue that has been shown in other ...
Random-sequence peptide libraries are a commonly used tool to identify novel ligands for binding antibodies, other proteins, and small molecules. It is often of interest to compare the selected peptide sequences to the natural protein binding partners to infer the exact binding site or the importan...
The method has been shown to tolerate peptides ranging from two to eight amino acids in length with a wide variety of functionality. We validated the capabilities of this system in a mock selection for the enrichment of a His-tagged DNA-scaffolded peptide phenotype from a library, which ...
A web tool for the design of prime-editing guide RNAs Article 28 September 2020 Peptide fusion improves prime editing efficiency Article Open access 18 June 2022 Main The efficient insertion of short DNA sequences into genomes could change the course of biotechnology and medicine1,2. Small ...
nascent peptides derived from the condensation of the activated amino acids are also trapped in the vesicles. 12 this led us to propose a general hypothesis that the seed sequences for life arose from short amyloid-forming peptides that were selected for in a prebiotic synthesis inside of vesic...
We report a method for the simultaneous determination of the sequence and absolute configuration of peptide amino acids using a combination of Edman degradation and HPLC–MS/CD. Phenylthiohydantoin (PTH) derivatives of 20 pairs of standard d- and l-amino