We demonstrate why this is so for cross-linguistic comparison with the following example in Seneca, a member of the Northern Iroquoian branch of the Iroquoian language family spoken in Northeast America32. The first line includes a word in the language, that is, a unit of meaning whose unit-...
In uninduced conditions, the BL21 strain is already expressing the recombinant silk gene, meaning that subsequent additions of IPTG may overwhelm cellular machinery beyond its ability to handle toxic protein expression, causing severe plasmid loss and low titers. Likewise, since the pLysS, SoluBL21,...