seqkit subseq --gtf Arabidopsis_thaliana.TAIR10.49.gtf.gz Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz -u 3 |head # 仅提取上游序列,如提取启动子区2k:-f仅定位不输出位置序列,-u输出上游序列,此处示例3bp seqkit subseq --gtf Arabidopsis_thaliana.TAIR10.49.gtf.gz Arabidopsis_thaliana.TAIR10.dna....
seqkit subseq --gtf Arabidopsis_thaliana.TAIR10.49.gtf.gz Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz -u 3 |head # 仅提取上游序列,如提取启动子区2k:-f仅定位不输出位置序列,-u输出上游序列,此处示例3bp seqkit subseq --gtf Arabidopsis_thaliana.TAIR10.49.gtf.gz Arabidopsis_thaliana.TAIR10.dna....
seqkit subseq --gtf Arabidopsis_thaliana.TAIR10.49.gtf.gz Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz -u 3 -f |head sliding 滑窗提取序列,支持环状基因组 #-s 步长为3,-W 序列长度为6个碱基 echo -e ">seq\nACGTacgtNN" | seqkit sliding -s 3 -W 6 ...
改变随机种子 2.3 subseq 用此指令提取序列. 可以观察到第一个参数是源文件,第二个参数是对应键名文件,我们根据name.list去提取文件. seqtk subseq genome.fa name.list | less -N 我们可以改变name.list的文件内容,让subseq提取不同位置的碱基.代码保持不变,获得的碱基不同了. ...
seqkit, add amino acid code O (pyrrolysine) and U (selenocysteine). seqkit replace, add flag --nr-width to fill leading 0s for {nr}, useful for preparing sequence submission (">strain_00001 XX", ">strain_00002 XX"). seqkit subseq, require BED file to be tab-delimited.SeqKit...