is a highly conserved GTP binding protein family and is considered to be the fourth cytoskeletal protein after microtubules, microfilaments and intermediate fibers. Septin proteins of pathogenic fungi are involved in cell polarity determination, morphological shaping and morphological transformation associated ...
Septin folding is mediated by the cytosolic chaperonin-containing TCP-1 (CCT), which also controls the folding of actin and tubulin, and septin degradation involves E3 ubiquitin ligases including parkin, the von Hippel–Lindau (VHL) tumor suppressor and the Ring Finger protein 8 (RNF8) (Craven ...
Azurin is a 14 kDa redox-active protein containing copper secreted by the bacterium Pseudomonas aeruginosa (Huang et al., 2020; Nar et al., 1992; Yaghoubi et al., 2020). Recently, this protein has aroused interest in the development of new treatments (Gammuto, Chiellini, Iozzo, Fani, & ...
1 µg/mL - 产品规格 种属反应 Human, Mouse 宿主/亚型 Rabbit / IgG 分类 Polyclonal 类型 Antibody 抗原 Recombinant fusion protein containing a sequence corresponding to amino acids 112-361 of human Septin 2 (NP_0043951) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 ...
(1.3 ± 0.1-fold) in the total amount of SEPT7 bound to bacteria in samples containing purified SEPT6ΔAH (Fig.3g, h). To test the role of the SEPT6 AH domain during infection, we infected HeLa cells producing msGFP–SEPT6WT or msGFP–SEPT6ΔAH withS. flexneriand quantified ...
The fractions containing the protein of interest were further purified by cation exchange chromatography. The pooled fractions were diluted with a 1:5 ratio to a final solution of 30 mM tris pH8, 100 mM NaCl, 1 mM EDTA and then incubated with 1 ml of a strong cation exchange resin (...
A. The septin protein structure consists of a GTP-binding domain composed of conserved motifs G1 (GxxxxGK[S/T]), G3 (DxxG) and the GTP-specificity motif G4 (xKxD). D, G, K, S, and T represent aspartic acid, glycine, lysine, serine, and threonine residues, respectively, and x ...
The first domain of the precursor protein encoded a 22 amino acid residue signal peptide and this displays around 90% similarity across the family [6,9]. These signal peptides were characterized by the conservation of most residues in the N-terminal sequence: Met-Ala-Phe-Leu-L...
The polybasic region of an individual septin monomer is brought into the proximity of a polybasic region of an adjacent septin protein upon interaction at the N- and C-terminal interface (Fig. 1A). In addition, most septins are predicted to have a C-terminal coiled coil domain. This region...
We used a reversed- phase column (SC-5ODS) and an electrochemical detector (ECD-300) in an HPLC system (EP-300, DG-300 and EPC-300, all from Eicom) with the mobile phase containing 0.1 M acetate–citric acid buffer and 0.19 g l À 1 sodium octyl sulfate. at pH 3.5, 16% CH3...