affinity chromatographyAn efficient method for the immobilization of DNA on Sephadex G200 in the presence of water soluble carboiimide is described and investigated in this paper. An increase in the extent of binding was observed when the incubation temperature of the DNA-Sephadex mixture was ...
1、葡聚糖凝胶sephadex g 25柱层析法脱盐分离蛋白质 实验技术总结,引言 层析法也称色谱法,是1906年俄国植物学家michael等发现并命名的。他将植物叶子的色素通过装填有吸附剂的柱子,各种色素以不同的速率流动后形成不同的色带而被分开,由此得名为“色谱法”(chromatography) 。后来无色物质也可利用吸附柱层析分离。
Sephadex LH-20 层析原理和应用基础
Chromatography, GelChemistry, ClinicalPermeabilitySerum and urine from patients with heavy proteinuria were analysed into their constituent proteins of equivalent molecular size on a column of Sephadex G 200. Urine to serum (U/P) ratios for each tube show steadily increasing ratios with diminishing ...
Sephadex LH-20 层析原理和应用基础
Sephadex LH-20 层析原理和应用基础